Integrin acts upstream of netrin signaling to regulate formation of the anchor cell's invasive membrane in C. elegans.
Integrin acts upstream of netrin signaling to regulate formation of the anchor cell's invasive membrane in C. elegans.
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DOI：
10.1016/j.devcel.2009.06.006
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发表时间：
2009-08
影响因子：
11.8
通讯作者：
Sherwood, David R.
中科院分区：
文献类型：
--
作者：
Hagedorn, Elliott J.;Yashiro, Hanako;Ziel, Joshua W.;Ihara, Shinji;Wang, Zheng;Sherwood, David R.
整合素的表达和活性与发育和病理过程密切相关，这些过程涉及通过基底膜的细胞侵袭。然而，整合素在介导这些侵袭中的作用仍不清楚。利用遗传学和可视化的锚细胞（AC）入侵C。elegans，我们最近已经表明netrin信号定向一个专门的侵入细胞膜结构域对基底膜。在这里，我们证明了整合素异源二聚体INA-1/PAT-3在AC侵袭中起着至关重要的作用，部分是通过将netrin受体α-40（DCC）靶向AC的质膜。对侵袭性膜组分磷脂酰肌醇4，5-二磷酸、Rac GTAT 1 -2和F-肌动蛋白的分析进一步表明，INA-1/PAT-3在促进这些分子的质膜结合中起着广泛的作用。总之，这些研究揭示了整合素在调节质膜靶向和netrin依赖性定向的专门侵入性膜结构域中的作用。
Integrin expression and activity have been strongly correlated with developmental and pathological processes involving cell invasion through basement membranes. The role of integrins in mediating these invasions, however, remains unclear. Utilizing the genetically and visually accessible model of anchor cell (AC) invasion in C. elegans, we have recently shown that netrin signaling orients a specialized invasive cell membrane domain towards the basement membrane. Here, we demonstrate that the integrin heterodimer INA-1/PAT-3 plays a crucial role in AC invasion, in part, by targeting the netrin receptor UNC-40 (DCC) to the AC’s plasma membrane. Analyses of the invasive membrane components phosphatidylinositol 4,5-bisphosphate, the Rac GTPase MIG-2 and F-actin further indicate that INA-1/PAT-3 plays a broad role in promoting the plasma membrane association of these molecules. Taken together, these studies reveal a role for integrin in regulating the plasma membrane targeting and netrin-dependent orientation of a specialized invasive membrane domain.
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影响因子：
7.5
作者：
Delon, Isabelle;Brown, Nicholas H.
通讯作者：
Brown, Nicholas H.
DOI：
10.1073/pnas.0709810105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Fitamant, Julien;Guenebeaud, Celine;Mehlen, Patrick
通讯作者：
Mehlen, Patrick
影响因子：
15.3
作者：
MARLIN, SD;MORTON, CC;SPRINGER, TA
通讯作者：
SPRINGER, TA
影响因子：
16.2
作者：
Gitai, Z;Yu, TW;Bargmann, CI
通讯作者：
Bargmann, CI
影响因子：
64.8
作者：
Kamath, RS;Fraser, AG;Ahringer, J
通讯作者：
Ahringer, J