Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness.
Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness.
复制标题
DOI：
10.1016/j.actbio.2013.04.001
复制
发表时间：
2013-07
影响因子：
9.7
通讯作者：
Yeh, A. T.
中科院分区：
文献类型：
--
作者：
Lee, P. -F.;Bai, Y.;Smith, R. L.;Bayless, K. J.;Yeh, A. T.
关键词：
在血管生成过程中，内皮细胞（EC）使用可溶性和不溶性的线索来扩大现有的血管网络，以满足组织不断变化的营养需求。这种扩张的基础是EC和细胞外基质（ECM）之间的物理相互作用，影响芽迁移，管腔形成和稳定。这些物理相互作用表明，ECM的机械性能可能会影响发芽EC，因此，血管生成反应。在三维血管生成模型中，单层EC被诱导侵入底层胶原基质，通过用微生物转氨酶（mTG）诱导胶原交联来测量作为胶原基质硬度的函数的血管生成反应。通过双轴机械测试，用mTG处理和孵育时间测量更硬的胶原基质。使用双光子激发荧光（TPF）和二次谐波产生（SHG），结果表明，胶原TPF强度增加与mTG治疗，和TPF/SHG的比例与双轴测试的机械刚度。SHG和OCM进一步用于显示其他ECM物理性质，如孔隙率和孔径不随mTG处理而改变，从而验证了基质刚度独立于基质密度而调节。结果表明，较硬的基质促进更多的血管生成芽侵入更深。在对照和mTG硬化基质之间没有观察到管腔大小的差异，但是使用SHG和OCM在更硬的凝胶中揭示了更大的重塑。这项研究的结果表明，血管生成反应的刚度的影响，并建议ECM的性能可能是有用的再生医学应用工程血管生成。
During angiogenesis, endothelial cells (ECs) use both soluble and insoluble cues to expand the existing vascular network to meet the changing trophic needs of the tissue. Fundamental to this expansion are physical interactions between ECs and extracellular matrix (ECM) that influence sprout migration, lumen formation and stabilization. These physical interactions suggest that ECM mechanical properties may influence sprouting ECs and, therefore, angiogenic responses. In a three-dimensional angiogenic model in which a monolayer of ECs is induced to invade an underlying collagen matrix, angiogenic responses were measured as a function of collagen matrix stiffness by inducing collagen crosslinking with microbial transglutaminase (mTG). By biaxial mechanical testing, stiffer collagen matrices were measured with both mTG treatment and incubation time. Using two-photon excited fluorescence (TPF) and second harmonic generation (SHG), it was shown that collagen TPF intensity increased with mTG treatment, and the TPF/SHG ratio correlated with biaxially tested mechanical stiffness. SHG and OCM were further used to show that other ECM physical properties such as porosity and pore size did not change with mTG treatment, thus verifying that matrix stiffness was tuned independently of matrix density. The results showed that stiffer matrices promote more angiogenic sprouts that invade deeper. No differences in lumen size were observed between control and mTG stiffened matrices, but greater remodeling was revealed in stiffer gels using SHG and OCM. The results of this study show that angiogenic responses are influenced by stiffness and suggest that ECM properties may be useful in regenerative medicine applications to engineer angiogenesis.
登录
查看更多内容
影响因子：
5.6
作者：
Gobeaux, F.;Mosser, G.;Belamie, E.
通讯作者：
Belamie, E.
影响因子：
--
作者：
Fereol, Sophie;Fodil, Redouane;Planus, Emmanuelle
通讯作者：
Planus, Emmanuelle
DOI：
10.1115/1.429652
发表时间：
2000-06-01
影响因子：
1.7
作者：
Girton, TS;Oegema, TR;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
4.8
作者：
Hiiragi, T;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S