Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS.
Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS.
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DOI：
10.3390/molecules22122147
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发表时间：
2017-12-04
期刊：
影响因子：
--
通讯作者：
Lee DY
中科院分区：
文献类型：
--
作者：
Lee JW;Choi BR;Kim YC;Choi DJ;Lee YS;Kim GS;Baek NI;Kim SY;Lee DY
人参皂苷的有效生产和利用，由于其独特的药理作用，正受到越来越多的关注。由于人参不同部位的人参皂苷含量不同，我们想对人参的根、叶、茎和浆果中的人参皂苷含量进行评估和比较。人参皂苷的提取采用70%(v/v)甲醇。采用优化的超高效液相色谱-四极杆飞行时间质谱仪(UPLC-QTOF/MS)对人参不同部位的人参皂苷成分进行了分析。然后对数据集进行多变量分析，包括主成分分析(PCA)和层次聚类分析(HCA)。建立了带有内部文库的UPLC-QTOF/MS方法，对58种人参皂苷进行了分析。用该方法成功地鉴定和定量了人参根、叶、茎和浆果中的39种人参皂苷。主成分分析和主成分分析对不同部位的人参皂苷成分进行了表征。并对各部位人参皂苷的含量进行了定量测定。本研究结果表明，UPLC-QTOF/MS方法可以作为人参不同部位人参皂苷成分分析的有效手段。
The effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, leave, stems, and berries. To extract the ginsenosides, 70% (v/v) methanol was used. The optimal ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) method was used to profile various ginsenosides from the different parts of P. ginseng. The datasets were then subjected to multivariate analysis including principal component analysis (PCA) and hierarchical clustering analysis (HCA). A UPLC-QTOF/MS method with an in-house library was constructed to profile 58 ginsenosides. With this method, a total of 39 ginsenosides were successfully identified and quantified in the ginseng roots, leave, stem, and berries. PCA and HCA characterized the different ginsenosides compositions from the different parts. The quantitative ginsenoside contents were also characterized from each plant part. The results of this study indicate that the UPLC-QTOF/MS method can be an effective tool to characterize various ginsenosides from the different parts of P. ginseng.
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