Expression and secretion of rat SPAM1(2B1 or PH-20) in the epididymis: role of testicular lumicrine factors.
Expression and secretion of rat SPAM1(2B1 or PH-20) in the epididymis: role of testicular lumicrine factors.
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附睾中大鼠SPAM1（2B1或PH-20）的表达和分泌：睾丸发光因子的作用。
DOI：
10.1016/j.matbio.2003.11.010
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Martin-DeLeon,PatriciaA
中科院分区：
文献类型：
--
作者：
Zhang,Hong;Jones,Roy;Martin-DeLeon,PatriciaA
大鼠精子表面抗原精子粘附分子 1，SPAM1（又名 2B1 或 PH-20）是一种质膜结合糖蛋白，具有透明质酸酶活性并在受精过程中发挥推定作用。以前该抗原被认为是睾丸特异性的，但最近显示它是在附睾（小鼠、猕猴和人类）中合成的。使用传出导管结扎（EDL）大鼠作为模型来产生无精子雄激素维持的附睾，我们检查了调节附睾2B1表达的因素。 RT-PCR 和原位转录杂交 (ISH) 研究表明，2B1 mRNA 在附睾所有三个区域的主要细胞中转录。还通过蛋白质印迹分析在正常附睾的无精子胞质中检测到其同源蛋白，并发现其经过内切蛋白水解分裂成两个大小与精子结合形式相似的亚基。使用 2B1 单克隆抗体进行的免疫组织化学证实该蛋白存在于附睾上皮和管腔分泌物中。无精子的 EDL 附睾中的染色强度比正常（有精子）附睾中的染色强度强得多。该蛋白质在中性 pH 值下具有透明质酸酶活性，并且其数量和活性在 EDL 附睾中似乎更高。有人提出可溶形式的SPAM1糖蛋白在附睾中合成和释放，除了雄激素之外，其调节可能涉及小管上皮和发光因子之间的串扰，后者可能源自睾丸。
Rat sperm surface antigen Sperm Adhesion Molecule1, SPAM1 (a.k.a. 2B1 or PH-20) is a plasma membrane-bound glycoprotein with hyaluronidase activity and putative roles during fertilization. Previously the antigen was thought to be testis-specific but recently it has been shown to be synthesized in the epididymis (mouse, macaque and human). Using the efferent ductule ligated (EDL) rat as a model to produce a sperm-free androgen-maintained epididymis, we have examined the factors regulating the expression of epididymal 2B1. RT-PCR and in situ transcript hybridization (ISH) studies showed that 2B1 mRNA is transcribed in the principal cells in all three regions of the epididymis. Its cognate protein was also detected by Western blot analysis in sperm-free cytosols from normal epididymis and found to undergo endoproteolytic cleavage into 2 subunits of similar size to the sperm-bound form. Immunohistochemistry with a monoclonal antibody to 2B1 confirmed that the protein is present in the epididymal epithelium and luminal secretions. The intensity of staining was much stronger in the sperm-free EDL epididymis than that in the normal (sperm-present) epididymis. The protein was shown to have hyaluronidase activity at neutral pH and both its quantity and activity appeared to be greater in the EDL epididymis. It is suggested that a soluble form of SPAM1 glycoprotein is synthesized and released in the epididymis and that in addition to androgens, its regulation may involve a cross-talk between the tubule epithelium and lumicrine factors, the latter possibly of testicular origin.
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影响因子：
1.7
作者：
D. Einon;M. Morgan
通讯作者：
M. Morgan
DOI：
--
发表时间：
1974
期刊：
Journal of Comparative and Physiological Psychology
影响因子：
--
作者：
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通讯作者：
Jay S. Rosenblatt
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DOI：
10.1037/h0025821
发表时间：
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期刊：
Journal of comparative and physiological psychology
影响因子：
--
作者：
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通讯作者：
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影响因子：
3.5
作者：
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DOI：
10.1037/h0076440
发表时间：
1975
期刊：
Journal of Comparative and Physiological Psychology
影响因子：
--
作者：
L. Paul;J. Kupferschmidt
通讯作者：
J. Kupferschmidt