Expression of a Grape VqSTS36-Increased Resistance to Powdery Mildew and Osmotic Stress in Arabidopsis but Enhanced Susceptibility to Botrytis cinerea in Arabidopsis and Tomato.
Expression of a Grape VqSTS36-Increased Resistance to Powdery Mildew and Osmotic Stress in Arabidopsis but Enhanced Susceptibility to Botrytis cinerea in Arabidopsis and Tomato.
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DOI：
10.3390/ijms19102985
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发表时间：
2018-09-30
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Huang L;Yin X;Sun X;Yang J;Rahman MZ;Chen Z;Wang X
芪合酶基因在提高植物对生物和非生物胁迫的耐受性方面发挥着重要作用。然而，这些STS基因介导的机制仍不清楚。为了深入了解STS基因防御生物和非生物胁迫的作用，我们通过农杆菌介导的转化在拟南芥和番茄（Micro-Tom）中过表达VqSTS 36。VqSTS 36转化的拟南芥品系表现出对白粉病的抗性增加，但VqSTS 36转化的拟南芥和番茄品系都表现出对灰葡萄孢的易感性增加。此外，转基因拟南芥植株在种子和幼苗中表现出对盐和干旱胁迫的耐受性。当转基因植物用不同的胁迫处理时，转基因拟南芥和番茄中防御相关基因的qPCR检测表明，VqSTS 36在不同的植物激素相关途径中发挥特定的作用，包括水杨酸，茉莉酸和脱落酸信号传导途径。所有这些结果提供了一个更好的理解VqSTS 36在生物和非生物胁迫的作用背后的机制。
Stilbene synthase genes make a contribution to improving the tolerances of biotic and abiotic stress in plants. However, the mechanisms mediated by these STS genes remain unclear. To provide insight into the role of STS genes defense against biotic and abiotic stress, we overexpressed VqSTS36 in Arabidopsis thaliana and tomato (Micro-Tom) via Agrobacterium-mediated transformation. VqSTS36-transformed Arabidopsis lines displayed an increased resistance to powdery mildew, but both VqSTS36-transformed Arabidopsis and tomato lines showed the increased susceptibility to Botrytis cinerea. Besides, transgenic Arabidopsis lines were found to confer tolerance to salt and drought stress in seed and seedlings. When transgenic plants were treated with a different stress, qPCR assays of defense-related genes in transgenic Arabidopsis and tomato suggested that VqSTS36 played a specific role in different phytohormone-related pathways, including salicylic acid, jasmonic acid, and abscisic acid signaling pathways. All of these results provided a better understanding of the mechanism behind the role of VqSTS36 in biotic and abiotic stress.
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影响因子：
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作者：
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通讯作者：
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