Dynamic PET and Optical Imaging and Compartment Modeling using a Dual-labeled Cyclic RGD Peptide Probe.
Dynamic PET and Optical Imaging and Compartment Modeling using a Dual-labeled Cyclic RGD Peptide Probe.
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作者：
Zhu L;Guo N;Li Q;Ma Y;Jacboson O;Lee S;Choi HS;Mansfield JR;Niu G;Chen X
目的：本研究的目的是确定动态光学成像是否可以提供与近红外染料/64 Cu双标记环状RGD肽的动态PET成像相当的动力学参数。研究方法：将整合素αvβ3结合RGD肽与大环螯合剂1，4，7，10-四氮杂环十二烷-1，4，7，10-四乙酸（DOTA）缀合用于铜标记和PET成像，并与近红外染料ZW-1缀合用于光学成像。通过细胞染色和受体结合试验表征RGD-C（DOTA）-ZW-1的体外生物学活性。在MDA-MB-435肿瘤模型上获得60分钟动态PET和光学成像。采用奇异值分解（SVD）方法从二维光学投影图像中计算动态光信号。利用计算机模型对动态光学和PET数据进行定量分析和比较。结果：双标记探针64 Cu-RGD-C（DOTA）-ZW-1在体内外均能与整合素特异性结合。由动态光学成像得到的结合电位（Bp）（1.762 ± 0.020）与由动态PET得到的结合电位（1.752 ± 0.026）相当。结论：利用奇异值分解的信号去混处理提高了二维动态光学数据动力学建模的准确性。我们的研究结果表明，在临床前异种移植模型中，二维动态光学成像与SVD分析可以实现与动态PET成像相当的定量结果。
Purpose: The aim of this study is to determine if dynamic optical imaging could provide comparable kinetic parameters to that of dynamic PET imaging by a near-infrared dye/64Cu dual-labeled cyclic RGD peptide. Methods: The integrin αvβ3 binding RGD peptide was conjugated with a macrocyclic chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) for copper labeling and PET imaging and a near-infrared dye ZW-1 for optical imaging. The in vitro biological activity of RGD-C(DOTA)-ZW-1 was characterized by cell staining and receptor binding assay. Sixty-min dynamic PET and optical imaging were acquired on a MDA-MB-435 tumor model. Singular value decomposition (SVD) method was applied to compute the dynamic optical signal from the two-dimensional optical projection images. Compartment models were used to quantitatively analyze and compare the dynamic optical and PET data. Results: The dual-labeled probe 64Cu-RGD-C(DOTA)-ZW-1 showed integrin specific binding in vitro and in vivo. The binding potential (Bp) derived from dynamic optical imaging (1.762 ± 0.020) is comparable to that from dynamic PET (1.752 ± 0.026). Conclusion: The signal un-mixing process using SVD improved the accuracy of kinetic modeling of 2D dynamic optical data. Our results demonstrate that 2D dynamic optical imaging with SVD analysis could achieve comparable quantitative results as dynamic PET imaging in preclinical xenograft models.
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影响因子：
3.1
作者：
Keereweer, Stijn;Kerrebijn, Jeroen D. F.;van Driel, Pieter B. A. A.;Xie, Bangwen;Kaijzel, Eric L.;Snoeks, Thomas J. A.;Que, Ivo;Hutteman, Merlijn;van der Vorst, Joost R.;Mieog, J. Sven D.;Vahrmeijer, Alexander L.;van de Velde, Cornelis J. H.;de Jong, Robert J. Baatenburg;Lowik, Clemens W. G. M.
通讯作者：
Lowik, Clemens W. G. M.
影响因子：
11.2
作者：
Chen, XY;Conti, PS;Moats, RA
通讯作者：
Moats, RA
影响因子：
6.3
作者：
LOGAN, J;FOWLER, JS;CHRISTMAN, DR
通讯作者：
CHRISTMAN, DR
影响因子：
12.4
作者：
Millard M;Odde S;Neamati N
通讯作者：
Neamati N
影响因子：
15.9
作者：
la Fougere, Christian;Suchorska, Bogdana;Tonn, Joerg-Christian
通讯作者：
Tonn, Joerg-Christian