PIR-B-deficient mice are susceptible to Salmonella infection.
PIR-B-deficient mice are susceptible to Salmonella infection.
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PIR-B缺乏小鼠易受沙门氏菌感染的影响。
DOI：
10.4049/jimmunol.181.6.4229
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发表时间：
2008-09-15
期刊：
影响因子：
--
通讯作者：
Kubagawa H
中科院分区：
文献类型：
--
作者：
Torii I;Oka S;Hotomi M;Benjamin WH Jr;Takai T;Kearney JF;Briles DE;Kubagawa H
配对的免疫球蛋白样受体激活（PIR-A）和抑制（PIR-B）异构体在许多造血细胞中表达，包括B淋巴细胞和骨髓细胞。为了确定PIR-A和PIR-B在原发性细菌感染中的功能作用，将PIR-B缺陷（PIR-B-/-）小鼠和野生型（WT）对照小鼠静脉注射一种减毒的肠炎鼠伤寒沙门氏菌（WB335）。PIR-B-/-小鼠比WT小鼠更容易感染沙门氏菌，其死亡率高，肝脏和脾脏细菌负荷高，并且不能清除循环中的细菌。虽然两组小鼠的主要细胞因子和沙门氏菌特异性抗体的血液水平大致相当，但在感染后7至14天，在肝脏中发现了不同的炎症病变模式：PIR-B-/-小鼠沿窦状动脉弥漫性扩散，而WT小鼠则是结节性局限性定位。感染后第14天，PIR-B-/-小鼠肝脏炎症细胞增多，脾脏B细胞和CD8+ T细胞减少。与WT BMMφ相比，PIR-B-/-骨髓源性巨噬细胞（BMMφ）在体外未能控制沙门氏菌的细胞内复制，部分原因是吞噬体氧化剂产生效率低下。PIR-B-/- BMMφ在暴露于沙门氏菌后也比WT BMMφ产生更多的亚硝酸盐和TNFα。这些发现表明，PIR-A和PIR-B平衡的破坏影响了它们在宿主防御细菌感染中的调节作用。
Paired immunoglobulin-like receptors of activating (PIR-A) and inhibitory (PIR-B) isoforms are expressed by many hematopoietic cells including B lymphocytes and myeloid cells. To determine the functional roles of PIR-A and PIR-B in primary bacterial infection, PIR-B-deficient (PIR-B-/-) and wild-type (WT) control mice were injected intravenously with an attenuated strain of Salmonella enterica Typhimurium (WB335). PIR-B-/- mice were found to be more susceptible to Salmonella infection than WT mice as evidenced by high mortality rate, high bacterial loads in the liver and spleen, and a failure to clear bacteria from the circulation. While blood levels of major cytokines and Salmonella-specific antibodies were mostly comparable in the two groups of mice, distinct patterns of inflammatory lesions were found in their livers at 7- to 14-d post-infection: diffuse spreading along the sinusoids in PIR-B-/- mice versus nodular restricted localization in WT mice. PIR-B-/- mice have more inflammatory cells in the liver but fewer B cells and CD8+ T cells in the spleen than WT mice at 14-d post-infection. PIR-B-/- bone marrow-derived macrophages (BMMφ) failed to control intracellular replication of Salmonella in vitro, in part due to inefficient phagosomal oxidant production, when compared with WT BMMφ. PIR-B-/- BMMφ also produced more nitrite and TNFα upon exposure to Salmonella than WT BMMφ. These findings suggest that the disruption of PIR-A and PIR-B balance affects their regulatory roles in host defense to bacterial infection.
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配对的免疫球蛋白样受体PIR-A和PIR-B的生化性质和细胞分布。
影响因子：
15.3
作者：
Kubagawa, H;Chen, C C;Ho, L H;Shimada, T S;Gartland, L;Mashburn, C;Uehara, T;Ravetch, J V;Cooper, M D
通讯作者：
Cooper, M D
影响因子：
15.9
作者：
JOHNSTON, RB;KEELE, BB;RAJAGOPALAN, KV
通讯作者：
RAJAGOPALAN, KV
影响因子：
8
作者：
Maeda, A;Scharenberg, AM;Kurosaki, T
通讯作者：
Kurosaki, T
影响因子：
6.4
作者：
Huycke, MM;Joyce, W;Wack, MF
通讯作者：
Wack, MF
DOI：
10.1073/pnas.95.5.2446
发表时间：
1998-03-03
影响因子：
11.1
作者：
Bléry, M;Kubagawa, H;Vivier, E
通讯作者：
Vivier, E