Fos protein expression induced by intracerebroventricular injection of vasopressin in unconditioned and conditioned mice
Fos protein expression induced by intracerebroventricular injection of vasopressin in unconditioned and conditioned mice
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脑室内注射加压素诱导未适应和适应小鼠中 Fos 蛋白表达
DOI：
10.1016/s0006-8993(99)01232-9
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
B. Soumireu
中科院分区：
文献类型：
--
作者：
V. Paban;B. Alescio;C. Devigne;B. Soumireu
精氨酸加压素（AVP）已被证明可以改善各种记忆任务中的记忆巩固。我们以前的研究已经指出海马参与了小鼠辨别性学习过程中的记忆巩固和提取过程。本研究试图确定除了海马以外，还有哪些脑区可能参与侧脑室注射（i.c.v.）注射AVP对视觉辨别任务中的记忆巩固的影响，使用针对Fos和Fos样蛋白的多克隆抗体。对于行为测试，在最后一次学习会话后以2 ng的行为活性剂量i.c.v.注射AVP，并在保持会话中评估巩固过程的改善。Fos和Fos样蛋白表达的变化在非条件和条件小鼠中测定。在非条件反射的小鼠中，2 ng剂量的AVP i.c.v.注射诱发了齿状回（DG）、海马CA1和CA3区、外侧隔（LS）、终纹床核、基底外侧核和中央杏仁核中Fos和Fos样蛋白表达的时间依赖性增加，在大多数这些脑区注射后120 min达到峰值。与此相反，在条件反射小鼠中，Fos表达水平的增加，评估120分钟后结束的学习和注射AVP，检测到只有在DG，腹侧CA3海马区，和LS。因此，在训练后注射AVP后观察到的模式与单独使用AVP诱发的模式不同，因为在单独使用AVP后激活的边缘系统结构中，只有DG、CA3海马区和LS似乎参与了AVP对辨别性学习记忆巩固的增强作用。
Arginine8–vasopressin (AVP) has been shown to improve memory consolidation in various mnemonic tasks. Our previous studies have pointed out the involvement of the hippocampus in memory consolidation and retrieval processes during discriminative learning by mice. The present study attempts to determine what other brain areas besides the hippocampus might be involved in the enhancing effect of intracerebroventricularly (i.c.v.) injected AVP on memory consolidation in a visual discrimination task using a polyclonal antibody that acts against Fos and Fos-like proteins. For behavioral testing, AVP was i.c.v. injected at the behaviorally active dose of 2 ng after the last learning session and improvement in consolidation processes was assessed in a retention session. Changes in Fos and Fos-like protein expression were determined in non-conditioned and conditioned mice. In non-conditioned mice, AVP i.c.v. injected at a dose of 2 ng evoked a time-dependent increase in Fos and Fos-like protein expression in the dentate gyrus (DG), CA1 and CA3 hippocampal fields, lateral septum (LS), bed nucleus of the stria terminalis, and basolateral and central amygdaloid nuclei, with a peak 120 min after the injection in most of the these brain areas. In contrast, in conditioned mice, an increase in the level of Fos expression, assessed 120 min after the end of learning and the injection of AVP, was detected only in the DG, ventral CA3 hippocampal field, and LS. Thus, the pattern observed after post-training injection of AVP was not the same as that evoked by AVP alone, since among the limbic structures activated following AVP alone, only the DG, the CA3 hippocampal field, and the LS seem to be involved in the enhancing effect of AVP on memory consolidation in discriminative learning.
DOI：
10.1073/pnas.90.8.3329
发表时间：
1993-04-15
影响因子：
11.1
作者：
GUTHRIE, KM;ANDERSON, AJ;GALL, C
通讯作者：
GALL, C
加压素和催产素拮抗剂的发现、开发和一些用途。
DOI：
--
发表时间：
1989
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Manning,M;Sawyer,WH
通讯作者：
Sawyer,WH
外周注射精氨酸 8-加压素可增加特定大脑区域的 Fos。
影响因子：
5
作者：
Wu,PH;Lança,AJ;Liu,JF;Man,CF;Kalant,H
通讯作者：
Kalant,H