Regulation of progestin receptors in medial amygdala: estradiol, phytoestrogens and sex.
Regulation of progestin receptors in medial amygdala: estradiol, phytoestrogens and sex.
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DOI：
10.1016/j.physbeh.2009.02.023
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发表时间：
2009-05-25
影响因子：
2.9
通讯作者：
Rissman, E. F.
中科院分区：
文献类型：
--
作者：
Kudwa, A. E.;Harada, N.;Honda, S. -I.;Rissman, E. F.
关键词：
在关键发育期和成年期暴露于雌激素会影响大脑的性别差异。我们研究了雌二醇（E2）和植物雌激素的作用，以及它们之间的相互作用，对大脑的潜在性别差异。我们使用芳香酶敲除（ArKO）小鼠，其不能产生内源性雌激素，沿着野生型（WT）同窝出生。小鼠在富含植物雌激素的饮食或几乎不含大豆来源的植物雌激素的饮食中妊娠、饲养和维持。切除成年雄性和雌性的性腺并接受填充有17-β-雌二醇的植入物以诱导孕酮受体（PR），而对照接受空植入物。5天后处死小鼠，并对含有背后内侧杏仁核（MePD）的脑切片进行PR免疫反应性处理。激活PR的性别差异需要成人E2治疗。高植物雌激素的饮食需要表达的性别差异PR后E2治疗。我们的数据强调的重要贡献，饮食植物雌激素的发展性别差异PR-IR在成年小鼠内侧杏仁核。我们推测，雄激素芳构化为雌激素和额外的雌激素的饮食来源是啮齿动物大脑性别差异的正常要求的一部分。
PHYSIOL BEH Exposure to estrogens during critical developmental periods and in adulthood affects sex differences in the brain. We examined the roles of estradiol (E2) and phytoestrogens, and their interactions, on potential sex differences in brain. We used aromatase knockout (ArKO) mice, which cannot produce endogenous estrogens, along with wild type (WT) littermates. Mice were gestated, raised and maintained on a diet either rich in phytoestrogens or a diet virtually void of soy-derived phytoestrogens. Adult males and females were gonadectomized and received implants filled with 17-β-estradiol to induce progestin receptors (PR), while controls received empty implants. Mice were sacrificed five days later and brain sections containing the posterodorsal medial amygdala (MePD) were processed for PR immunoreactivity. Activation of sex differences in PR required adult E2 treatment. A diet high in phytoestrogens was required for expression of sex differences in PR after E2 treatment. Our data underscore the important contribution of dietary phytoestrogens for the development of sex differences in PR-ir in the adult mouse medial amygdala. We hypothesize that both aromatization of androgens to estrogens and dietary sources of additional estrogens are part of the normal requirement for sex differences in the rodent brain.
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影响因子：
4.8
作者：
Bodo, C;Kudwa, AE;Rissman, EF
通讯作者：
Rissman, EF
影响因子：
4.8
作者：
Kuiper, GGJM;Lemmen, JG;Gustafsson, JÄ
通讯作者：
Gustafsson, JÄ
影响因子：
3.2
作者：
Jacob, DA;Temple, JL;Rissman, EF
通讯作者：
Rissman, EF
影响因子：
5.3
作者：
Cooke, BM;Woolley, CS
通讯作者：
Woolley, CS
影响因子：
4.8
作者：
Gréco, B;Allegretto, EA;Blaustein, JD
通讯作者：
Blaustein, JD