Noninvasive monitoring of hepatic glutathione depletion through fluorescence imaging and blood testing.
Noninvasive monitoring of hepatic glutathione depletion through fluorescence imaging and blood testing.
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DOI：
10.1126/sciadv.abd9847
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发表时间：
2021-03
期刊：
影响因子：
13.6
通讯作者：
Zheng J
中科院分区：
文献类型：
--
作者：
Jiang X;Zhou Q;Du B;Li S;Huang Y;Chi Z;Lee WM;Yu M;Zheng J
肝谷胱甘肽耗竭是一种重要的病理生理学事件，现在可以轻松监测以进行早期疾病治疗。肝脏谷胱甘肽在调节整个身体的氧化还原电位方面发挥着关键作用，已知其消耗会增加对许多疾病所涉及的氧化应激的易感性。然而，这种关键的病理生理学事件只能通过高端仪器进行无创检测，或者通过手术活检进行侵入性检测，这限制了氧化应激相关疾病的临床前研究和临床预防。在这里，我们报告说，体内荧光成像和血液检测（临床一线检测）都可以在体内可清除的纳米探针的帮助下，以高特异性和准确性用于非侵入性连续监测肝谷胱甘肽的消耗，其中发射和表面化学物质被肝窦中的肝谷胱甘肽选择性激活和转化。这些发现为设计外源性血液标记物开辟了一条新途径，这些标记物可以通过特定的纳米生化相互作用将局部疾病的信息带回血流，以便轻松快速地检测疾病。
Hepatic glutathione depletion, a crucial pathophysiological event, can now be readily monitored for early disease remedy. Hepatic glutathione plays a key role in regulating redox potential of the entire body, and its depletion is known to increase susceptibility to oxidative stress involved in many diseases. However, this crucial pathophysiological event can only be detected noninvasively with high-end instrumentation or invasively with surgical biopsy, limiting both preclinical research and clinical prevention of oxidative stress–related diseases. Here, we report that both in vivo fluorescence imaging and blood testing (the first-line detection in the clinics) can be used for noninvasive and consecutive monitoring of hepatic glutathione depletion at high specificity and accuracy with assistance of a body-clearable nanoprobe, of which emission and surface chemistries are selectively activated and transformed by hepatic glutathione in the liver sinusoids. These findings open a new avenue to designing exogenous blood markers that can carry information of local disease through specific nanobiochemical interactions back to the bloodstream for facile and rapid disease detection.
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影响因子：
15
作者：
Cheng, Penghui;Miao, Qingqing;Pu, Kanyi
通讯作者：
Pu, Kanyi
影响因子：
38.3
作者：
Jiang, Xingya;Du, Bujie;Zheng, Jie
通讯作者：
Zheng, Jie
影响因子：
25.7
作者：
Lee, AU;Farrell, GC
通讯作者：
Farrell, GC
DOI：
10.1007/s004330050100
发表时间：
1998-12-01
期刊：
RESEARCH IN EXPERIMENTAL MEDICINE
影响因子：
--
作者：
Purucker, E;Winograd, R;Matern, S
通讯作者：
Matern, S
影响因子：
13.5
作者：
LAUTERBURG, BH;ADAMS, JD;MITCHELL, JR
通讯作者：
MITCHELL, JR