Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos
Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos
复制标题
非洲爪蟾胚胎中三种间隙连接 mRNA 水平的差异调节
作者：
R L Gimlich;N. M. Kumar;N. Gilula
通过用克隆的哺乳动物间隙连接 cDNA 对 cDNA 文库进行低严格筛选，已鉴定出可能编码卵母细胞和早期胚胎中间隙连接蛋白的非洲爪蟾 mRNA。这些 mRNA 的水平显示出显着不同的时间调节和组织分布。使用旨在强调不同间隙连接基因产物的重要结构相似性的命名法，推导的多肽被指定为非洲爪蟾α1和α2间隙连接蛋白。 α2 间隙连接 mRNA 是母体转录物，在原肠胚晚期消失。除卵巢外，在成人的任何器官中均未检测到它，并且主要（如果不是全部）存在于卵母细胞和早期胚胎中。 α1 间隙连接 mRNA 在器官发生过程中出现，并在多种器官的 RNA 中检测到。它也存在于完全生长的卵母细胞中，但在体内和体外的卵母细胞成熟后迅速降解。 α 1 和 α 2 mRNA 编码的蛋白质与哺乳动物心脏的主要间隙连接亚基（连接蛋白 43）具有不同程度的氨基酸序列相似性。结合我们之前关于胚胎中期 (β1) 间隙连接 mRNA 的报告，结果表明卵母细胞生长和受精后发育过程中的细胞间通讯是一个复杂的现象，涉及多个基因的协调调节。
Xenopus mRNAs that potentially encode gap junction proteins in the oocyte and early embryo have been identified by low-stringency screening of cDNA libraries with cloned mammalian gap junction cDNAs. The levels of these mRNAs show strikingly different temporal regulation and tissue distribution. Using a nomenclature designed to stress important structural similarities of distinct gap junction gene products, the deduced polypeptides have been designated the Xenopus alpha 1 and alpha 2 gap junction proteins. The alpha 2 gap junction mRNA is a maternal transcript that disappears by the late gastrula stage. It is not detected in any organ of the adult except the ovary, and resides primarily, if not exclusively, in the oocytes and early embryos. The alpha 1 gap junction mRNA appears during organogenesis, and is detected in RNA from a wide variety of organs. It is also found in full-grown oocytes, but is rapidly degraded upon oocyte maturation, both in vivo and in vitro. The alpha 1 and alpha 2 mRNAs encode proteins with different degrees of amino acid sequence similarity to the predominant gap junction subunit of the mammalian heart (connexin 43). Together with our earlier report of a mid-embryonic (beta 1) gap junction mRNA, the results suggest that intercellular communication during oocyte growth and postfertilization development is a complex phenomenon involving the coordinated regulation of several genes.
影响因子：
2.7
作者：
Weir,MP;Lo,CW
通讯作者：
Lo,CW
对分离的完整大鼠肝脏间隙连接和间隙连接衍生的单膜结构中主要蛋白质的拓扑分析。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Zimmer,DB;Green,CR;Evans,WH;Gilula,NB
通讯作者：
Gilula,NB