Analysis of the conservation of synteny between Fugu and human chromosome 12
Analysis of the conservation of synteny between Fugu and human chromosome 12
复制标题
河豚与人类12号染色体同线性保守性分析
作者：
A. Montpetit;M. Wilson;M. Chevrette;B. Koop;D. Sinnett
背景红鳍河豚（Fugu rubripes）基因组结构紧凑，越来越被认为是比较基因组研究的重要脊椎动物模型。特别是，人类和河豚基因组之间的保守同线性的大区域表明其实用性，以确定致病基因。人类染色体12p12经常在各种血液恶性肿瘤和实体瘤中缺失，但实际的肿瘤抑制基因仍然unidentified.ResultsWe调查了大约200 kb的基因组区域周围的ETV6基因座在河豚（fETV6），以找到保守的功能特征，如基因或调控区，这可以让我们深入了解人类癌细胞中被删除的基因的性质。在fETV6基因座附近鉴定了7个基因。我们发现，与人类12号染色体的同线性是保守的，但广泛的基因组重排之间发生的河豚和人类ETV6 locus.ConclusionThis比较分析导致识别以前未知的基因在人类基因组中的一些潜在的重要调控序列以及。这是一个很好的迹象表明，紧凑的河豚基因组的分析将是有价值的，以确定在整个脊椎动物的进化保守的功能特征。
BackgroundThe pufferfish Fugu rubripes (Fugu) with its compact genome is increasingly recognized as an important vertebrate model for comparative genomic studies. In particular, large regions of conserved synteny between human and Fugu genomes indicate its utility to identify disease-causing genes. The human chromosome 12p12 is frequently deleted in various hematological malignancies and solid tumors, but the actual tumor suppressor gene remains unidentified.ResultsWe investigated approximately 200 kb of the genomic region surrounding the ETV6 locus in Fugu (fETV6) in order to find conserved functional features, such as genes or regulatory regions, that could give insight into the nature of the genes targeted by deletions in human cancer cells. Seven genes were identified near the fETV6 locus. We found that the synteny with human chromosome 12 was conserved, but extensive genomic rearrangements occurred between the Fugu and human ETV6 loci.ConclusionThis comparative analysis led to the identification of previously uncharacterized genes in the human genome and some potentially important regulatory sequences as well. This is a good indication that the analysis of the compact Fugu genome will be valuable to identify functional features that have been conserved throughout the evolution of vertebrates.
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影响因子：
14.9
作者：
Yang, CR;Yeh, SY;Boothman, DA
通讯作者：
Boothman, DA
儿童急性淋巴细胞白血病：12 号染色体短臂上 TEL 和 KIP1 区域两个不同的缺失区域的鉴定。
影响因子：
20.3
作者：
Takeuchi,S;Bartram,CR;Miller,CW;Reiter,A;Seriu,T;Zimmerann,M;Schrappe,M;Mori,N;Slater,J;Miyoshi,I;Koeffler,HP
通讯作者：
Koeffler,HP
鉴定出进化上保守的 110 个碱基对顺式作用调节序列，该序列控制小鼠神经板中的 Wnt-1 表达。
DOI：
10.1242/dev.125.14.2735
发表时间：
1998
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Rowitch,DH;Echelard,Y;Danielian,PS;Gellner,K;Brenner,S;McMahon,AP
通讯作者：
McMahon,AP
影响因子：
20.3
作者：
K. Stegmaier;Shona Pendse;G. Barker;P. Bray-Ward;David C. Ward;Kate T Montgomery;Kenneth S. Krauter;Carol Reynolds;Jeffrey Sklar;Mia Donnelly;Stefan K. Bohlander;Janet D. Rowley;Stephen E. Sallan;D. Gilliland;Todd R. Golub;Bosron Ma
通讯作者：
K. Stegmaier;Shona Pendse;G. Barker;P. Bray-Ward;David C. Ward;Kate T Montgomery;Kenneth S. Krauter;Carol Reynolds;Jeffrey Sklar;Mia Donnelly;Stefan K. Bohlander;Janet D. Rowley;Stephen E. Sallan;D. Gilliland;Todd R. Golub;Bosron Ma
DOI：
10.1073/pnas.96.17.9745
发表时间：
1999-08-17
影响因子：
11.1
作者：
Hukriede, NA;Joly, L;Ekker, M
通讯作者：
Ekker, M