Inhibition of acid-induced apoptosis by targeting ASIC1a mRNA with short hairpin RNA
Inhibition of acid-induced apoptosis by targeting ASIC1a mRNA with short hairpin RNA
复制标题
通过短发夹 RNA 靶向 ASIC1a mRNA 抑制酸诱导的细胞凋亡
DOI：
10.1111/j.1745-7254.2007.00627.x
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发表时间：
2007-10
期刊：
影响因子：
--
通讯作者：
Jian-quan Zheng
中科院分区：
文献类型：
--
作者：
Qing-e Jin;Xiao-yun Ma;Xie-chuan Weng;Jian-quan Zheng
目的：研究酸敏感离子通道（acid-sensing ion channel，ASIC）1a在细胞外酸诱导的C6胶质瘤细胞死亡和凋亡中的作用，方法：利用RNA干扰技术建立稳定的ASIC 1a沉默C6胶质瘤细胞系，通过RT-PCR和Western blot分析证实ASIC 1a沉默的C6胶质瘤细胞系。用乳酸脱氢酶（LDH）和3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）测定分析酸暴露后的细胞活力。结果：稳定转染短发夹状RNA后ASIC 1a蛋白表达下调，可降低细胞死亡率，提高细胞活力。ASIC 1a沉默的细胞系中的凋亡率低于野生型C6细胞系。ASIC 1a沉默的C6细胞亚G 0期细胞比例低于野生型细胞。结论：细胞外酸通过ASIC 1a机制诱导C6胶质瘤细胞死亡和凋亡。
AbstractAim:To study the role of acid-sensing ion channel (ASIC) 1a in the cell death and apoptosis induced by extracellular acid in C6 glioma cells.Methods:The stable ASIC1a-silenced C6 cell line, built with RNA interference technology, were confirmed by RT-PCR and Western blot analysis. The cell viability following acid exposure was analyzed with lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The apoptotic cells dyed with Annexin-V and propidium iodide were measured with a flow cytometer, while the changes of cell cycle were also assayed.Results:The downregulation of ASIC1a proteins by stable transfection of short hairpin RNA decreased the cell death percentage and increased cell viability following acid exposure with LDH and the MTT assay. The rate of apoptosis was lower in the ASIC1a-silenced cell line than that in the wild-type C6 cell line. The percentage of sub-G0 cells was lower in the ASIC1a-silenced C6 cells than that in the wild-type cells.Conclusion:Extracellular acid induced cell death and apoptosis via ASIC1a mechanisms in the C6 glioma cells.
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影响因子：
2.9
作者：
X. Weng;Jian-quan Zheng;Xiao-dan Gai;Jin Li;W. Xiao
通讯作者：
X. Weng;Jian-quan Zheng;Xiao-dan Gai;Jin Li;W. Xiao
影响因子：
5.5
作者：
de la Rosa, DA;Krueger, SR;Canessa, CM
通讯作者：
Canessa, CM
影响因子：
--
作者：
BOBIK, A;GROOMS, A;GRINPUKEL, S
通讯作者：
GRINPUKEL, S
DOI：
10.1523/jneurosci.23-13-05496.2003
发表时间：
2003-07
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
J. Wemmie;C. Askwith;E. Lamani;M. Cassell;J. Freeman;M. Welsh
通讯作者：
J. Wemmie;C. Askwith;E. Lamani;M. Cassell;J. Freeman;M. Welsh
影响因子：
3.3
作者：
KRISHTAL, OA;PIDOPLICHKO, VI
通讯作者：
PIDOPLICHKO, VI