Attenuated stress response to acute lipopolysaccharide challenge and ethanol administration in vasopressin V1b receptor knockout mice.
Attenuated stress response to acute lipopolysaccharide challenge and ethanol administration in vasopressin V1b receptor knockout mice.
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DOI：
10.1111/j.1365-2826.2007.01560.x
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发表时间：
2007-07
影响因子：
3.2
通讯作者：
O'Carroll AM
中科院分区：
文献类型：
--
作者：
Lolait SJ;Stewart LQ;Roper JA;Harrison G;Jessop DS;Young WS 3rd;O'Carroll AM
存在于垂体前叶促肾上腺皮质激素上的精氨酸抗利尿激素（Avp） 1b受体（Avpr1b）参与促肾上腺皮质激素（ACTH）分泌的刺激，尤其是在应激时。促肾上腺皮质激素释放激素（Crh）被认为是急性应激时主要的促肾上腺皮质激素（ACTH）分泌剂，而Avp似乎是慢性应激情况下下丘脑-垂体-肾上腺（HPA）轴反应的更主要介质。为了研究Avpr1b在HPA轴对急性应激反应中的作用，我们测量了Avpr1b基因敲除（KO）小鼠和野生型对照组在细菌脂多糖（LPS）攻击和乙醇（EtOH）处理下的ACTH和皮质酮（CORT）血浆水平。缺乏Avpr1b的小鼠对急性LPS（30分钟）的血浆ACTH和CORT反应明显降低，但正常的ACTH和CORT对更长的免疫系统激活剂暴露（4小时）有反应。与野生型小鼠相比，Avpr1b KO小鼠的血浆ACTH和CORT水平在麻醉、镇静剂量（3.2和4g/kg）的刺激下显著降低。雌性Avpr1b野生型小鼠血浆ACTH和CORT分泌明显高于雄性Avpr1b野生型小鼠。Avpr1b KO和野生型小鼠在急性EtOH给药后血液酒精水平（BALs）没有差异。我们的研究结果清楚地表明，Avpr1b在HPA轴对急性免疫应激和EtOH中毒的反应中起着重要作用。
The arginine vasopressin (Avp) 1b receptor (Avpr1b) present on anterior pituitary corticotrophs is involved in the stimulation of adrenocorticotrophin (ACTH) secretion, especially during times of stress. Corticotrophin-releasing hormone (Crh) is considered the major ACTH secretagogue during acute stress while Avp appears to be the more dominant mediator of the hypothalamic-pituitary-adrenal (HPA) axis response during chronic stress situations. To investigate the role of the Avpr1b in the HPA axis response to acute stress, we measured ACTH and corticosterone (CORT) plasma levels in Avpr1b knockout (KO) mice and wild-type controls in response to bacterial lipopolysaccharide (LPS) challenge and ethanol (EtOH) administration. Mice deficient in Avpr1b had markedly compromised plasma ACTH and CORT responses to acute (30 min) LPS, but normal ACTH and CORT response to more extended exposure (4 h) to the immune system activator. The plasma ACTH and CORT levels stimulated by intoxicating, sedative doses of EtOH (3.2 and 4g/kg) were significantly decreased in the Avpr1b KO mice, as compared to wild-type littermates. Significantly higher EtOH-induced plasma ACTH and CORT secretion was measured in female than in male Avpr1b wild-type mice. There was no difference in the blood alcohol levels (BALs) following acute EtOH administration in Avpr1b KO or wild-type mice of either gender. Our results clearly suggest that the Avpr1b plays a significant role in the HPA axis response to acute immune stress and EtOH intoxication.
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影响因子：
4.8
作者：
Battaglia, DF;Brown, ME;Karsch, FJ
通讯作者：
Karsch, FJ
影响因子：
3.2
作者：
Keeney, A;Jessop, DS;Blackburn-Munro, RE
通讯作者：
Blackburn-Munro, RE
影响因子：
6.1
作者：
COLBERN, DL;TENHAAF, J;GREIDANUS, TBV
通讯作者：
GREIDANUS, TBV
DOI：
10.1073/pnas.0600875103
发表时间：
2006-05-16
影响因子：
11.1
作者：
Koshimizu, Taka-aki;Nasa, Yoshihisa;Tsujimoto, Gozoh
通讯作者：
Tsujimoto, Gozoh
影响因子：
4.1
作者：
ENGLER, D;PHAM, T;CLARKE, IJ
通讯作者：
CLARKE, IJ