Cytochrome b₅ reductase-cytochrome b₅ as an active P450 redox enzyme system in Phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to CYP63A2.
Cytochrome b₅ reductase-cytochrome b₅ as an active P450 redox enzyme system in Phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to CYP63A2.
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DOI：
10.1016/j.abb.2011.02.023
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发表时间：
2011-05-01
影响因子：
3.9
通讯作者：
Yadav JS
中科院分区：
文献类型：
--
作者：
Syed K;Kattamuri C;Thompson TB;Yadav JS
Two central redox enzyme systems exist to reduce eukaryotic P450 enzymes, the P450 oxidoreductase (POR) and the cyt b5 reductase-cyt b5. In fungi, limited information is available for the cytb5 reductase-cyt b5 system. Here we characterized the kinetic mechanism of (cyt b5r)-cyt b5 redox system from the model white-rot fungus Phanerochaete chrysosporium (Pc) and made a quantitative comparison to the POR system. We determined that Pc-cyt b5r followed a “ping-pong” mechanism and could directly reduce cytochrome c. However, unlike other cyt b5 reductases, Pc-cyt b5r lacked the typical ferricyanide reduction activity, a standard for cyt b5 reductases. Through co-expression in yeast, we demonstrated that the Pc-cyt b5r-cyt b5 complex is capable of transferring electrons to Pc-P450 CYP63A2 for its benzo(a)pyrene monooxygenation activity and that the efficiency was comparable to POR. In fact, both redox systems supported oxidation of an estimated one-third of the added benzo(a)pyrene amount. To our knowledge, this is the first report to indicate that the cyt b5r-cyt b5 complex of fungi is capable of transferring electrons to a P450 monooxygenase. Furthermore, this is the first eukaryotic quantitative comparison of the two P450 redox enzyme systems (POR and cyt b5r-cyt b5) in terms of supporting a P450 monooxygenase activity.
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影响因子：
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作者：
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DOI：
10.1016/s1357-2725(98)00099-5
发表时间：
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期刊：
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影响因子：
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通讯作者：
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影响因子：
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