The effects of galanin-like peptide on energy balance, body temperature and brain activity in the mouse and rat are independent of the GALR2/3 receptor.
The effects of galanin-like peptide on energy balance, body temperature and brain activity in the mouse and rat are independent of the GALR2/3 receptor.
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DOI：
10.1111/j.1365-2826.2007.01625.x
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发表时间：
2008-01
影响因子：
3.2
通讯作者：
Lawrence CB
中科院分区：
文献类型：
--
作者：
Man PS;Lawrence CB
甘丙肽样肽（GALP）是一种神经肽，被认为作用于甘丙肽受体GALR 1，GALR 2和GALR 3。在大鼠中，i. c. v.注射GALP对能量平衡具有二分作用，在第一个小时内刺激进食，但在24小时内减少摄食量和体重，并导致核心体温升高。 在小鼠中，GALP仅诱导体温升高，其对核心体温的影响尚不清楚。本研究的一个目的是确定GALP对小鼠核心体温的影响。侧脑室注射GALP对清醒小鼠的摄食量无影响，但在24 h时摄食量和体重显著降低。 它还导致核心体温立即下降，随后体温升高。为了了解GALP对小鼠与大鼠能量平衡的这些不同影响，并确定GALR 2和GALR 3的参与，进行免疫组织化学以定位c-Fos，细胞活化的标志物。侧脑室注射GALP诱导小鼠和大鼠脑室周围实质、脑室室管膜细胞和脑膜中c-Fos表达。GALP还诱导大鼠视上核、下丘脑背内侧核、下丘脑外侧核和孤束核中的c-Fos表达，但在小鼠中不诱导。在大鼠中中枢给予GALR 2/3激动剂不会在GALP注射后表达该蛋白的任何脑区中诱导c-Fos，并且对大鼠或小鼠的食物摄入量、体重和体温没有影响。这些数据表明，与大鼠相比，GALP对小鼠的能量平衡和脑活动产生不同的影响，这不太可能是由于GALR 2或GALR 3受体的激活。
Galanin‐like peptide (GALP) is a neuropeptide that is thought to act on the galanin receptors GALR1, GALR2 and GALR3. In rats, i.c.v. injection of GALP has dichotomous actions on energy balance, stimulating feeding over the first hour, but reducing food intake and body weight at 24 h, as well as causing an increase in core body temperature. In mice, GALP only induces an anorexic action, and its effects on core body temperature are unknown. One aim of the present study was to determine the effects of GALP on core body temperature in mice. Intracerebroventricular injection of GALP into conscious mice had no effect on feeding over 1 h, but caused a significant reduction in food intake and body weight at 24 h. It also caused an immediate drop in core body temperature, which was followed by an increase in body temperature. To understand these different effects of GALP on energy balance in mice compared to rats, and to determine the involvement of GALR2 and GALR3, immunohistochemistry was performed to localise c‐Fos, a marker of cell activation. Intracerebroventricular injection of GALP induced c‐Fos expression in the parenchyma surrounding the ventricles, the ventricular ependymal cells and the meninges in mice and rats. GALP also induced c‐Fos expression in the supraoptic nucleus, dorsomedial hypothalamic nucleus, lateral hypothalamus and nucleus tractus solitarius in rats but not in mice. Central administration of a GALR2/3 agonist in rats did not induce c‐Fos in any of the brain regions that expressed this protein after GALP injection, and had no effect on food intake, body weight and body temperature in rats or mice. These data suggest that GALP induces differential effects on energy balance and brain activity in mice compared to rats, which are unlikely to be due to activation of the GALR2 or GALR3 receptor.
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影响因子：
4.7
作者：
Elliott-Hunt CR;Pope RJ;Vanderplank P;Wynick D
通讯作者：
Wynick D
影响因子：
3.2
作者：
Kageyama, H;Kita, T;Shioda, S
通讯作者：
Shioda, S
DOI：
10.1073/pnas.161293598
发表时间：
2001-08-14
影响因子：
11.1
作者：
Liu, HX;Brumovsky, P;Hökefelt, T
通讯作者：
Hökefelt, T
影响因子：
3.5
作者：
Kauffman, AS;Buenzle, J;Rissman, EF
通讯作者：
Rissman, EF
影响因子：
3.3
作者：
Hohmann, JG;Juréus, A;Steiner, RA
通讯作者：
Steiner, RA