Library construction for next-generation sequencing: overviews and challenges.
Library construction for next-generation sequencing: overviews and challenges.
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DOI：
10.2144/000114133
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发表时间：
2014
期刊：
影响因子：
2.7
通讯作者：
Ordoukhanian P
中科院分区：
文献类型：
--
作者：
Head SR;Komori HK;LaMere SA;Whisenant T;Van Nieuwerburgh F;Salomon DR;Ordoukhanian P
高通量测序，也称为下一代测序（NGS），已经彻底改变了基因组研究。近年来，NGS技术稳步发展，成本下降，测序应用的数量和范围呈指数级增长。在这里，我们研究了测序文库质量的关键作用，并考虑了从DNA和RNA来源制备NGS文库时的重要挑战。诸如RNA或DNA源材料的量和物理特性以及所需应用（即，基因组测序、靶向测序、RNA-seq、ChIP-seq、RIP-seq和甲基化）在制备高质量测序文库的背景下被解决。此外，还对目前从单细胞制备NGS文库的方法进行了讨论。
High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality and consider important challenges when preparing NGS libraries from DNA and RNA sources. Factors such as the quantity and physical characteristics of the RNA or DNA source material as well as the desired application (i.e., genome sequencing, targeted sequencing, RNA-seq, ChIP-seq, RIP-seq, and methylation) are addressed in the context of preparing high quality sequencing libraries. In addition, the current methods for preparing NGS libraries from single cells are also discussed.
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影响因子：
9.1
作者：
Grolleau-Julius, Annabelle;Ray, Donna;Yung, Raymond L.
通讯作者：
Yung, Raymond L.
影响因子：
64.5
作者：
Gifford CA;Ziller MJ;Gu H;Trapnell C;Donaghey J;Tsankov A;Shalek AK;Kelley DR;Shishkin AA;Issner R;Zhang X;Coyne M;Fostel JL;Holmes L;Meldrim J;Guttman M;Epstein C;Park H;Kohlbacher O;Rinn J;Gnirke A;Lander ES;Bernstein BE;Meissner A
通讯作者：
Meissner A
DOI：
10.1073/pnas.1101148108
发表时间：
2011-04-05
影响因子：
11.1
作者：
Cheng, Julong;Torkamani, Ali;Lerner, Richard A.
通讯作者：
Lerner, Richard A.
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
4.6
作者：
Bhargava, Vipul;Ko, Pang;Willems, Erik;Mercola, Mark;Subramaniam, Shankar
通讯作者：
Subramaniam, Shankar