Promyelocytic leukemia nuclear bodies associate with transcriptionally active genomic regions.
Promyelocytic leukemia nuclear bodies associate with transcriptionally active genomic regions.
复制标题
临时细胞性白血病核体与转录活性基因组区域相关。
DOI：
10.1083/jcb.200305142
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发表时间：
2004-02-16
期刊：
影响因子：
--
通讯作者：
Sheer D
中科院分区：
文献类型：
--
作者：
Wang J;Shiels C;Sasieni P;Wu PJ;Islam SA;Freemont PS;Sheer D
早幼粒细胞白血病(PML)蛋白聚集成与多种核过程相关的核小体。在这里，我们报告了一个基因座与其最近的PML小体之间的距离与该基因座周围的转录活性和基因密度相关。活跃的X染色体上的基因与PML小体的关联比它们在非活跃的X染色体上的沉默同源物更显著。我们还发现，一个仅在S期转录的组蛋白编码基因簇，与细胞周期的G0/G1期相比，与S期的PML小体有更强的相关性。然而，对几个基因的特定RNA转录本的可视化显示，PML小体本身并不是这些基因的转录部位。此外，通过RNA干扰下调PML小体并不会优先改变与PML小体密切相关的基因的表达。我们认为PML小体形成于高转录活性的核室，但它们不直接调节这些核室中基因的转录。
The promyelocytic leukemia (PML) protein is aggregated into nuclear bodies that are associated with diverse nuclear processes. Here, we report that the distance between a locus and its nearest PML body correlates with the transcriptional activity and gene density around the locus. Genes on the active X chromosome are more significantly associated with PML bodies than their silenced homologues on the inactive X chromosome. We also found that a histone-encoding gene cluster, which is transcribed only in S-phase, is more strongly associated with PML bodies in S-phase than in G0/G1 phase of the cell cycle. However, visualization of specific RNA transcripts for several genes showed that PML bodies were not themselves sites of transcription for these genes. Furthermore, knock-down of PML bodies by RNA interference did not preferentially change the expression of genes closely associated with PML bodies. We propose that PML bodies form in nuclear compartments of high transcriptional activity, but they do not directly regulate transcription of genes in these compartments.
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DOI：
10.1083/jcb.152.5.1099
发表时间：
2001-03-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boisvert FM;Kruhlak MJ;Box AK;Hendzel MJ;Bazett-Jones DP
通讯作者：
Bazett-Jones DP
DOI：
10.1083/jcb.145.6.1119
发表时间：
1999-06-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Croft JA;Bridger JM;Boyle S;Perry P;Teague P;Bickmore WA
通讯作者：
Bickmore WA
影响因子：
8
作者：
Carbone, R;Pearson, M;Pelicci, PG
通讯作者：
Pelicci, PG
影响因子：
21.3
作者：
Brown, KE;Amoils, S;Fisher, AG
通讯作者：
Fisher, AG
影响因子：
11.4
作者：
BORDEN, KLB;BODDY, MN;FREEMONT, PS
通讯作者：
FREEMONT, PS