The effect of diet on the fecal bacterial flora of mice and on their resistance to infection.
The effect of diet on the fecal bacterial flora of mice and on their resistance to infection.
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DOI：
10.1084/jem.115.6.1161
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发表时间：
1962-06-01
期刊：
影响因子：
--
通讯作者：
SCHAEDLER RW
中科院分区：
文献类型：
--
作者：
DUBOS RJ;SCHAEDLER RW
研究了某些饮食方案对小鼠粪便中乳杆菌植物群及其抗感染能力的影响。半合成饮食与纯化酪蛋白或小麦面筋作为唯一的蛋白质来源，引起了更少的数量的活乳酸杆菌在粪便中比其他饮食含有不明的天然产品，如目前在全麦和全脂牛奶的混合物，或在某些商业颗粒。此外，其中一种具有假根形态的类胡萝卜素从饲喂半合成饲料的动物粪便中完全消失。在动物从复合饲料转换到合成饲料后的几天内，肠道植物群的变化变得明显。而且，这种变化不完全可逆。然而，当动物从合成饲料转换回复合饲料时，乳酸杆菌的总数增加了，从粪便中完全消失的假根乳酸杆菌只是缓慢地或根本没有出现。在12个连续的实验中，三种在粪便中产生大量乳酸杆菌的饮食也使小鼠对金黄色葡萄球菌和肺炎克雷伯氏菌的实验感染具有比半合成饮食更高的抵抗力。然而，尚未获得直接证据表明这两种现象之间存在因果关系。内毒素给药后，喂食半合成酪蛋白饲料的小鼠肠球菌和大肠杆菌数量迅速大幅增加，但喂食颗粒饲料的小鼠肠球菌和大肠杆菌数量没有增加。在用另一群并非无病原体的小鼠进行的两个初步实验中，还发现在相同条件下，在喂食半合成酪蛋白饮食的动物中，类根型乳酸杆菌消失，而肠球菌和大肠杆菌的数量逐渐增加。无论将小鼠圈养在单独的金属丝网笼中，还是将小鼠分组在以木屑为垫料的较大笼中，饮食对肠道植物群和对实验感染的抵抗力的影响都同样明显。这是真的，即使床上用品每周只换一次，因此变得非常脏。
A study was made of the effect of certain dietary regimens on the lactobacillus flora in the stools of mice and on their resistance to infection. Semi-synthetic diets with purified casein or wheat gluten as sole source of protein, gave rise to much smaller numbers of viable lactobacilli in the stools than did other diets containing unidentified natural products—as present for example in mixtures of whole wheat and whole milk, or in certain commercial pellets. Furthermore, one of the lactobacillus types with rhizoid morphology disappeared completely from the stools of animals fed the semi-synthetic diet. The change in the lactobacillus flora became apparent within a very few days after the animals had been shifted from the complex to the synthetic diet Moreover, this change was not completely reversible. Whereas the total numbers of lactobacilli increased when the animals were shifted back from the synthetic to the complex diets, the rhizoid lactobacilli which had disappeared completely from the stools reappeared only slowly or not at all. In twelve consecutive experiments the three diets which gave rise to the large numbers of lactobacilli in the stools also conferred on the mice a much higher resistance to experimental infection with Staphylococcus aureus and Klebsiella pneumoniae, than did the semi-synthetic diets. However, direct evidence has not yet been obtained that the two kinds of phenomena were causally related. Following administration of endotoxin there was a rapid and very large increase in the numbers of enterococci and coliform bacilli in mice fed the semi-synthetic casein diet, but not in those fed the pellets. In two preliminary experiments carried out with another colony of mice, not pathogen-free, it was also found that the rhizoid type of lactobacilli disappeared from animals fed the semi-synthetic casein diet while enterococci and coliform bacilli progressively increased in numbers under the same conditions. The dietary effects on the lactobacillus flora, and on resistance to experimental infection, were equally pronounced whether the mice were housed in individual cages on wire grids, or grouped in larger cages with wood shavings as litter. This was true even if the bedding was changed only once weekly and became therefore grossly soiled.
DOI：
10.1084/jem.110.6.921
发表时间：
1959-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
SCHAEDLER RW;DUBOS RJ
通讯作者：
DUBOS RJ
影响因子：
15.3
作者：
SCHAEDLER, R W;DUBOS, R J
通讯作者：
DUBOS, R J