Tools for engineering coordinated system behaviour in synthetic microbial consortia.
Tools for engineering coordinated system behaviour in synthetic microbial consortia.
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DOI：
10.1038/s41467-018-05046-2
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发表时间：
2018-07-11
影响因子：
16.6
通讯作者：
Polizzi KM
中科院分区：
文献类型：
--
作者：
Kylilis N;Tuza ZA;Stan GB;Polizzi KM
将合成生物学推进到多细胞水平需要开发多个细胞间的通信通道，以最小的信号干扰传播信息。群体感应装置是构建具有协调系统行为的微生物群落的基石技术，其开发主要集中在同源酰基高丝氨酸内酯（阿勒）/转录因子对上，而使用非同源对作为设计特征受到的关注有限。在这里，我们展示了一个大型的AHL接收器设备库，所有同源和非同源化学信号相互作用量化，我们开发了一个软件工具，自动选择正交通信通道。我们使用这种方法来确定多达四个正交通道在硅片，并通过实验证明同时使用三个通道的共培养。多个非干扰细胞间通信通道的开发是一个有利的步骤，有助于设计用于分布式生物计算、提高生物过程效率、细胞专业化和空间组织等应用的合成财团。合成微生物群落的工程需要使用合成的，正交的细胞到细胞的communicationchannels. In这里，作者提出了一个库的特征AHL接收器设备和一个软件工具，用于自动识别非干扰化学通信通道。
Advancing synthetic biology to the multicellular level requires the development of multiple cell-to-cell communication channels that propagate information with minimal signal interference. The development of quorum-sensing devices, the cornerstone technology for building microbial communities with coordinated system behaviour, has largely focused on cognate acyl-homoserine lactone (AHL)/transcription factor pairs, while the use of non-cognate pairs as a design feature has received limited attention. Here, we demonstrate a large library of AHL-receiver devices, with all cognate and non-cognate chemical signal interactions quantified, and we develop a software tool that automatically selects orthogonal communication channels. We use this approach to identify up to four orthogonal channels in silico, and experimentally demonstrate the simultaneous use of three channels in co-culture. The development of multiple non-interfering cell-to-cell communication channels is an enabling step that facilitates the design of synthetic consortia for applications including distributed bio-computation, increased bioprocess efficiency, cell specialisation and spatial organisation. The engineering of synthetic microbial communities necessitates the use of synthetic, orthogonal cell-to-cell communication channels. Here the authors present a library of characterised AHL-receiver devices and a software tool for the automatic identification of non-interfering chemical communication channels.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
17.1
作者：
Courbet, Alexis;Endy, Drew;Bonnet, Jerome
通讯作者：
Bonnet, Jerome
DOI：
10.1073/pnas.92.14.6424
发表时间：
1995-07-03
影响因子：
11.1
作者：
OCHSNER, UA;REISER, J
通讯作者：
REISER, J
影响因子：
46.9
作者：
Bacchus, William;Lang, Moritz;Fussenegger, Martin
通讯作者：
Fussenegger, Martin
DOI：
10.1073/pnas.81.13.4154
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ENGEBRECHT, J;SILVERMAN, M
通讯作者：
SILVERMAN, M