Phosphorylation of Na(+)-H+ antiporter is not stimulated by phorbol ester and acidification in granulocytic HL-60 cells.
Phosphorylation of Na(+)-H+ antiporter is not stimulated by phorbol ester and acidification in granulocytic HL-60 cells.
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在粒细胞 HL-60 细胞中，佛波酯和酸化不会刺激 Na( )-H 逆向转运蛋白的磷酸化。
DOI：
10.1152/ajpcell.1993.264.5.c1278
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Berk,BC
中科院分区：
文献类型：
--
作者：
Rao,GN;Sardet,C;Pouysségur,J;Berk,BC
在HL-60细胞分化为粒细胞样细胞的过程中，Na(+)-H+逆向转运蛋白的mRNA和蛋白水平增加了10-15倍。然而，通过从酸负荷[细胞内pH(Phi)6.5]恢复来衡量功能活性只增加了大约两倍。此外，基础phi(在没有碳酸氢盐的情况下测量)从7.15增加到7.26，这表明在HL-60细胞分化过程中反向转运蛋白的“设定点”发生了变化。为了深入了解Na(+)-H+逆向转运蛋白在HL-60细胞分化中的作用，我们研究了NHE-1、NHE-3和NHE-4亚型的mRNA表达。在分化过程中，只有NHE-1亚型的mRNA表达增加。由于最近发现反向转运蛋白受磷酸化的调节，我们接下来研究了HL-60细胞分化过程中NHE-1蛋白的磷酸化。通过暴露在1微米维甲酸中分化6天，NHE-1蛋白的合成增加了15倍。然而，32P标记的反向转运蛋白的免疫沉淀显示条带强度降低。这些数据表明，在HL-60细胞分化过程中，尽管PHI增加，但NHE-1的磷酸化水平净下降。然而，分化细胞从酸负荷(phi 6.51)中恢复的速度明显快于对照组：分别为62+/-6和38+/-8 mmolH+.min-1.1细胞-1。然而，酸负荷使分化和未分化的HL-60细胞中的反向转运蛋白磷酸化减少了两倍。
During differentiation of HL-60 cells into granulocyte-like cells, mRNA and protein levels for the Na(+)-H+ antiporter increased 10- to 15-fold. However, functional activity, as measured by recovery from an acid load [intracellular pH (pHi) 6.5] increased by only about twofold. In addition, basal pHi (measured in the absence of bicarbonate) increased from 7.15 to 7.26, suggesting an alteration in the antiporter's "set point" during HL-60 cell differentiation. To gain insight into the role of the Na(+)-H+ antiporter in HL-60 cell differentiation, we studied mRNA expression of the NHE-1, NHE-3, and NHE-4 isoforms. Only the NHE-1 isoform mRNA increased during differentiation. Because it has recently been shown that the antiporter is regulated by phosphorylation, we next studied NHE-1 protein phosphorylation during HL-60 cell differentiation. Differentiation by exposure to 1 microM retinoic acid for 6 days caused a 15-fold increase in the synthesis of the NHE-1 protein. However, immunoprecipitation of 32P-labeled antiporter showed a decrease in band intensity. These data indicate that during HL-60 cell differentiation, there was a net decrease in the phosphorylation of NHE-1 despite an increase in pHi. Nonetheless, recovery from an acid load (pHi 6.51) was significantly more rapid in differentiated than control cells: 62 +/- 6 vs. 38 +/- 8 mmol H+.min-1.1 cells-1, respectively. However, acid loading decreased antiporter phosphorylation by twofold in differentiated and undifferentiated HL-60 cells.(ABSTRACT TRUNCATED AT 250 WORDS)
冈田酸是一种磷酸酶抑制剂，可诱导 Na /H 逆向转运的激活和磷酸化。
DOI：
10.1016/s0021-9258(18)98630-8
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Bianchini;M. Woodside;C. Sardet;J. Pouysségur;A. Takai;S. Grinstein
通讯作者：
S. Grinstein
中国仓鼠胚胎成纤维细胞转化后细胞内pH值升高。
DOI：
10.1073/pnas.84.9.2766
发表时间：
1987
影响因子：
11.1
作者：
Ober,SS;Pardee,AB
通讯作者：
Pardee,AB
佛波酯和趋化因子可诱导粒细胞（如 HL60 细胞）细胞质 Ca2 和 pH 的明显变化。
DOI：
--
发表时间：
1989
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
A. Ladoux;I. Krawice;C. Damais;C. Frelin
通讯作者：
C. Frelin