Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes.
Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes.
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DOI：
10.1186/1755-8794-4-42
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发表时间：
2011-05-16
影响因子：
2.7
通讯作者：
Kiyosawa H
中科院分区：
文献类型：
--
作者：
Saito R;Kohno K;Okada Y;Osada Y;Numata K;Kohama C;Watanabe K;Nakaoka H;Yamamoto N;Kanai A;Yasue H;Murata S;Abe K;Tomita M;Ohkohchi N;Kiyosawa H
最近的研究已经确定了成千上万的正义-反义基因对不同的基因组的cDNA序列的计算映射。这些研究表明，人类和小鼠基因组中约25%的所有转录单位参与顺式-正义-反义对。然而，已知的有义-反义对的数量仍然有限，因为目前可用的cDNA序列仅代表构成每种细胞类型的转录物组的转录物总数的一部分。为了发现编码在重要基因（如癌症相关基因）的反义链中的新型反义转录物，我们使用我们定制的微阵列平台沿着2376个探针进行反义转录物的表达分析，这些探针专门设计用于检测501个已知基因的潜在反义转录物，适用于癌症研究。使用从6名患者获得的结肠癌组织和癌组织周围的正常组织，我们发现没有poly（A）尾的反义转录物从大约80%的这些众所周知的基因表达。这一观察结果与我们先前的发现一致，即在细胞中表达的许多反义转录物是poly（A）-。我们还鉴定了101和71个反义探针，分别在正常组织和癌组织中特异性地显示高水平表达。我们的微阵列分析确定了新的反义转录与表达谱特异性的癌组织，其中一些可能发挥作用的调控网络潜在的肿瘤发生，因此是进一步的实验验证的潜在目标。我们的微阵列数据可在http://www.brc.riken.go.jp/ncrna2007/viewer-Saito-01/index.html上获得。
Recent studies have identified thousands of sense-antisense gene pairs across different genomes by computational mapping of cDNA sequences. These studies have shown that approximately 25% of all transcriptional units in the human and mouse genomes are involved in cis-sense-antisense pairs. However, the number of known sense-antisense pairs remains limited because currently available cDNA sequences represent only a fraction of the total number of transcripts comprising the transcriptome of each cell type. To discover novel antisense transcripts encoded in the antisense strand of important genes, such as cancer-related genes, we conducted expression analyses of antisense transcripts using our custom microarray platform along with 2376 probes designed specifically to detect the potential antisense transcripts of 501 well-known genes suitable for cancer research. Using colon cancer tissue and normal tissue surrounding the cancer tissue obtained from 6 patients, we found that antisense transcripts without poly(A) tails are expressed from approximately 80% of these well-known genes. This observation is consistent with our previous finding that many antisense transcripts expressed in a cell are poly(A)-. We also identified 101 and 71 antisense probes displaying a high level of expression specifically in normal and cancer tissues respectively. Our microarray analysis identified novel antisense transcripts with expression profiles specific to cancer tissue, some of which might play a role in the regulatory networks underlying oncogenesis and thus are potential targets for further experimental validation. Our microarray data are available at http://www.brc.riken.go.jp/ncrna2007/viewer-Saito-01/index.html.
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影响因子：
4.4
作者：
Numata K;Osada Y;Okada Y;Saito R;Hiraiwa N;Nakaoka H;Yamamoto N;Watanabe K;Okubo K;Kohama C;Kanai A;Abe K;Kiyosawa H
通讯作者：
Kiyosawa H
影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
DOI：
10.1126/science.1162253
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Seila AC;Calabrese JM;Levine SS;Yeo GW;Rahl PB;Flynn RA;Young RA;Sharp PA
通讯作者：
Sharp PA
影响因子：
4.4
作者：
Grigoriadis A;Oliver GR;Tanney A;Kendrick H;Smalley MJ;Jat P;Neville AM
通讯作者：
Neville AM