An Optimized Bacteriophage Cocktail Can Effectively Control Salmonella in vitro and in Galleria mellonella.
An Optimized Bacteriophage Cocktail Can Effectively Control Salmonella in vitro and in Galleria mellonella.
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优化的噬菌体鸡尾酒可以在体外和Mellonella Galleria中有效控制沙门氏菌。
DOI：
10.3389/fmicb.2020.609955
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发表时间：
2020
影响因子：
5.2
通讯作者：
Clokie MRJ
中科院分区：
文献类型：
--
作者：
Nale JY;Vinner GK;Lopez VC;Thanki AM;Phothaworn P;Thiennimitr P;Garcia A;AbuOun M;Anjum MF;Korbsrisate S;Galyov EE;Malik DJ;Clokie MRJ
沙门氏菌是人类肠胃炎的主要原因，主要通过受污染的家禽和猪肉感染。噬菌体可用于控制动物中的沙门氏菌感染，这可以在产品可供消费之前打破感染循环。在这里，21 myoviruses和siphovirus消除沙门氏菌在体外和体内的潜力进行了检查，目的是开发一种生物控制策略，以减少家禽和猪的感染。总的来说，沙门氏菌针对23个家禽和10个猪流行沙门氏菌血清型分离株进行了测试。尽管单个噬菌体在感染后6小时内显着减少了代表性分离株的细菌生长，但1小时后发生了细菌再生长，表明耐药菌株的增殖。为了减少噬菌体的耐药性，在体外开发了一种新型的三噬菌体混合物，并在优化的大蜡螟幼虫沙门氏菌感染模型中进行了进一步研究，该模型定植有代表性的猪、鸡和实验室菌株。对所有供试菌株，G.在细菌暴露（预防性方案）前102小时给予的大蜡螟幼虫存活，并且在噬菌体处理后24小时和整个实验时间（72小时）检测不到沙门氏菌。与细菌一起施用抗生素（共感染）或细菌暴露后2小时（补救方案）也改善了存活率（分别为73-100%和15- 88%），但不如预防性应用有效。这些家畜前的数据支持这种混合物的未来应用，以进一步开发有效治疗家禽和猪的沙门氏菌感染。未来的工作将集中在鸡尾酒配方，以确保稳定性和纳入饲料，并用于治疗目标动物的感染。
Salmonella spp. is a leading cause of gastrointestinal enteritis in humans where it is largely contracted via contaminated poultry and pork. Phages can be used to control Salmonella infection in the animals, which could break the cycle of infection before the products are accessible for consumption. Here, the potential of 21 myoviruses and a siphovirus to eliminate Salmonella in vitro and in vivo was examined with the aim of developing a biocontrol strategy to curtail the infection in poultry and swine. Together, the phages targeted the twenty-three poultry and ten swine prevalent Salmonella serotype isolates tested. Although individual phages significantly reduced bacterial growth of representative isolates within 6 h post-infection, bacterial regrowth occurred 1 h later, indicating proliferation of resistant strains. To curtail bacteriophage resistance, a novel three-phage cocktail was developed in vitro, and further investigated in an optimized Galleria mellonella larva Salmonella infection model colonized with representative swine, chicken and laboratory strains. For all the strains examined, G. mellonella larvae given phages 2 h prior to bacterial exposure (prophylactic regimen) survived and Salmonella was undetectable 24 h post-phage treatment and throughout the experimental time (72 h). Administering phages with bacteria (co-infection), or 2 h post-bacterial exposure (remedial regimen) also improved survival (73–100% and 15–88%, respectively), but was less effective than prophylaxis application. These pre-livestock data support the future application of this cocktail for further development to effectively treat Salmonella infection in poultry and pigs. Future work will focus on cocktail formulation to ensure stability and incorporation into feeds and used to treat the infection in target animals.
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影响因子：
4.5
作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
2011-12-02
影响因子：
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作者：
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