Selective Regulation of trkC Expression by NT3 in the Developing Peripheral Nervous System
Selective Regulation of trkC Expression by NT3 in the Developing Peripheral Nervous System
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NT3 在发育中的周围神经系统中选择性调节 trkC 表达
DOI：
10.1523/jneurosci.19-15-06559.1999
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Davies
中科院分区：
文献类型：
--
作者：
S. Wyatt;G. Middleton;E. Doxakis;A. Davies
我们研究了神经营养素-3（NT-3）对其受体酪氨酸激酶（trkC）表达的影响。编码全长和激酶缺陷型受体的trkC转录物的表达几乎完全限于三叉神经节中的神经元，并在整个发育过程中显着增加。在NT 3 +/−胚胎中，三叉神经节中trkC mRNA的水平远低于野生型胚胎，尽管神经节中神经元的总数没有显著减少。这表明内源性NT 3调节三叉神经元中trkC的表达，与群体大小的变化无关。在NT 3 −/−胚胎中，三叉神经节中的神经元数量远低于野生型胚胎，并且trkC mRNA的平均神经元水平进一步降低。还观察到培养的三叉神经元中trkC mRNA表达的直接调节，尽管发现无论NT 3的存在与否，在外植体培养物中trkC mRNA水平比在解离培养物中持续更好，表明trkC mRNA表达受神经节内的其他因素调节。与三叉神经元相反，在NT 3 −/−胚胎的交感神经链神经元中，trkC mRNA的水平维持在正常水平，并且在交感神经元培养物中，NT 3没有增加trkC mRNA的水平。发育中的皮肤组织中的TrkC mRNA表达也不受NT 3无效突变的影响。总之，我们的研究结果提供了第一个明确的证据表明，一个trk受体，酪氨酸激酶的表达，是由其在体内的配体的生理水平进行调节，并显示，NT 3的调节是细胞类型特异性的。
We have studied the influence of neurotrophin-3 (NT3) on the expression of its receptor tyrosine kinase, trkC, in embryonic mice. The expression of trkC transcripts encoding full-length and kinase-deficient receptors was almost entirely restricted to neurons in the trigeminal ganglion and increased markedly throughout development. In NT3+/− embryos, the level of trkC mRNA in the trigeminal ganglion was much lower than that in wild-type embryos, although there was no significant reduction in the total number of neurons in the ganglion. This demonstrates that endogenous NT3 regulates trkC expression in trigeminal neurons independently of changes in population size. InNT3−/− embryos, the number of neurons in the trigeminal ganglion was much lower than in wild-type embryos, and there was a further reduction in the mean neuronal level of trkC mRNA. Direct regulation of trkC mRNA expression in cultured trigeminal neurons was also observed, although the finding that trkC mRNA levels were sustained better in explant cultures than in dissociated cultures irrespective of the presence of NT3 suggests that trkC mRNA expression is regulated by additional factors within the ganglion. In contrast to trigeminal neurons, the level of trkC mRNA was sustained at normal levels in neurons of the sympathetic chain ofNT3−/− embryos and was not increased by NT3 in sympathetic neuron cultures. TrkC mRNA expression in developing cutaneous tissues was also unaffected by the NT3 null mutation. In summary, our findings provide the first clear evidence that the expression of a trk receptor, tyrosine kinase, is regulated by physiological levels of its ligand in vivo and show that regulation by NT3 is cell type-specific.
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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DOI：
10.1242/dev.126.10.2191
发表时间：
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期刊：
Development (Cambridge, England)
影响因子：
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作者：
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通讯作者：
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