Genetic effects of an air discharge plasma on Staphylococcus aureus at the gene transcription level

Genetic effects of an air discharge plasma on Staphylococcus aureus at the gene transcription level
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空气放电等离子体在基因转录水平对金黄色葡萄球菌的遗传效应

DOI:
10.1063/1.4921754
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发表时间:
2015-05
影响因子:
4
通讯作者:
Chu, Paul K.
Chu, Paul K.
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Xia, Weidong;Sun, Qiang;Cheng, Cheng;Chu, Paul K.

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采用实时定量聚合酶链反应(PCR)技术,研究了不同剂量的常压室温空气等离子体处理后金黄色葡萄球菌(Staphylococcus aureus)基因表达调控(转录水平)的动态变化。等离子体处理影响基因的转录,这些基因与细菌的环境应激抗性相关的几个重要生物分子过程相关,包括氧化应激反应、生物膜形成、抗生素抗性和DNA损伤保护/修复。由气相中的等离子体放电产生和/或在液相中诱导的反应性物质可以解释这些基因表达变化。
The dynamics of gene expression regulation (at transcription level) in Staphylococcus aureus after different doses of atmospheric-pressure room-temperature air plasma treatments are investigated by monitoring the quantitative real-time polymerase chain reaction. The plasma treatment influences the transcription of genes which are associated with several important bio-molecular processes related to the environmental stress resistance of the bacteria, including oxidative stress response, biofilm formation, antibiotics resistance, and DNA damage protection/repair. The reactive species generated by the plasma discharge in the gas phase and/or induced in the liquid phase may account for these gene expression changes.
DOI: 10.1088/1009-1963/15/7/028
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期刊: CHINESE PHYSICS
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