Murine CD14 gene expression in vivo: extramyeloid synthesis and regulation by lipopolysaccharide.
Murine CD14 gene expression in vivo: extramyeloid synthesis and regulation by lipopolysaccharide.
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鼠CD14基因在体内的表达：脂多糖的脂外合成和调节。
DOI：
10.1084/jem.181.3.857
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发表时间：
1995-03-01
影响因子：
15.3
通讯作者：
Loskutoff, David J.
中科院分区：
文献类型：
--
作者：
Fearns, Colleen;Kravchenko, Vladimir V.;Ulevitch, Richard J.;Loskutoff, David J.
采用小鼠模型系统研究体内CD 14基因表达的分布和调控。Western印迹分析未能检测到来自未处理的CB 6（BALB/c x C57 B16）小鼠的血浆中的CD 14，但显示来自用脂多糖（LPS）处理的小鼠的血浆中的CD 14水平显著增加。CD 14的血浆水平以时间和剂量依赖性方式增加，在8 - 16 h之间达到最大值。从小鼠组织中提取的总RNA的北方印迹分析显示，在未处理动物的许多组织中，CD 14 mRNA水平较低，但显著，在子宫、脂肪组织和肺中水平最高。腹腔注射LPS后，检测到所有器官中的CD 14基因表达的诱导，器官之间的诱导程度不同。CD 14 mRNA的诱导具有时间和剂量依赖性。在心脏和肺中的最大诱导在注射LPS后2-4 h观察到，而肝脏和肾脏在8 - 16 h之间显示最大诱导。原位杂交结果显示，CD 14 mRNA在许多组织的髓样细胞中表达，并且在这些细胞中的表达被LPS上调。出乎意料的是，在组织内的其他细胞中也检测到CD 14 mRNA，包括上皮细胞，并且这些细胞类型中的表达也被LPS上调。免疫化学分析显示，CD 14抗原共定位于表达CD 14 mRNA的细胞的细胞质。这些研究表明，CD 14基因表达不限于骨髓细胞，并且CD 14的表达水平受到暴露于LPS的影响。
A murine model system was used to study the distribution and regulation of CD14 gene expression in vivo. Western blot analysis failed to detect CD14 in plasma from untreated CB6 (BALB/c x C57Bl6) mice, but showed markedly increased levels of CD14 in plasma from mice treated with lipopolysaccharide (LPS). Plasma levels of CD14 increased in a time- and dose-dependent manner, reaching a maximum between 8 and 16 h. Northern blot analysis of total RNA extracted from mouse tissues revealed low, but significant, levels of CD14 mRNA in many tissues of untreated animals with the highest levels in uterus, adipose tissue, and lung. After intraperitoneal injection of LPS, induction of CD14 gene expression was detected in all organs examined with the extent of induction varying between organs. Induction of CD14 mRNA was both time and dose dependent. Maximum induction in the heart and lung was observed 2-4 h after injection of LPS, while liver and kidney showed maximal induction between 8 and 16 h. In situ hybridization showed that CD14 mRNA was expressed in myeloid cells in many tissues, and that expression in these cells was upregulated by LPS. Unexpectedly, CD14 mRNA was also detected in other cells within tissues, including epithelial cells, and expression in these cell types also was upregulated by LPS. Immunochemical analysis revealed that CD14 antigen colocalized to the cytoplasm of cells expressing CD14 mRNA. These studies demonstrate that CD14 gene expression is not restricted to myeloid cells, and that the level of expression of CD14 is influenced by exposure to LPS.
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DOI：
10.1073/pnas.90.21.9887
发表时间：
1993-11-01
影响因子：
11.1
作者：
READ, MA;CORDLE, SR;HAWIGER, J
通讯作者：
HAWIGER, J
影响因子：
32.4
作者：
PUGIN, J;HEUMANN, D;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
--
作者：
IKEWAKI, N;INOKO, H
通讯作者：
INOKO, H
DOI：
10.1084/jem.175.6.1697
发表时间：
1992-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lee JD;Kato K;Tobias PS;Kirkland TN;Ulevitch RJ
通讯作者：
Ulevitch RJ
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N