Ventrolateral Periaqueductal Gray Neurons Are Active During Urination.
Ventrolateral Periaqueductal Gray Neurons Are Active During Urination.
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DOI：
10.3389/fncel.2022.865186
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发表时间：
2022
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
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腹外侧导水管周围灰质（VLPAG）被认为是膀胱控制的主要PAG柱。PAG神经元（尤其是VLPAG神经元）和支配膀胱逼尿肌的脑桥排尿中心（PMC）中的神经元具有解剖学和功能性突触连接。关于膀胱的神经控制的流行观点是PAG神经元接收关于上游脑区做出的排尿决定的信息，并且因此通过它们的直接投射激活PMC以启动排尿反射。然而，PMC投射VLPAG神经元的确切位置，它们对排尿的反应以及它们的全脑输入仍然不清楚。在这里，我们确定了分布的VLPAG神经元，可能参与控制膀胱或项目的PMC通过逆行神经追踪。群体钙离子信号PMC投射VLPAG神经元高度相关的膀胱收缩和排尿所示，在自由活动的动物体内记录。使用基于RV的逆行跨突触追踪策略，形态学结果表明，排尿相关的PMC投射VLPAG神经元接收来自多个排尿相关的高级脑区，如内侧视前区，内侧前额叶皮质和外侧下丘脑的密集输入。因此，我们的研究结果揭示了一个新的见解VLPAG控制膀胱功能，并提供了一个潜在的治疗神经源性膀胱功能障碍的中脑节点。
The ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. PAG neurons (especially VLPAG neurons) and neurons in the pontine micturition center (PMC) innervating the bladder detrusor have anatomical and functional synaptic connections. The prevailing viewpoint on neural control of the bladder is that PAG neurons receive information on the decision to void made by upstream brain regions, and consequently activate the PMC through their direct projections to initiate urination reflex. However, the exact location of the PMC-projecting VLPAG neurons, their activity in response to urination, and their whole-brain inputs remain unclear. Here, we identified the distribution of VLPAG neurons that may participate in control of the bladder or project to the PMC through retrograde neural tracing. Population Ca2+ signals of PMC-projecting VLPAG neurons highly correlated with bladder contractions and urination as shown by in vivo recording in freely moving animals. Using a RV-based retrograde trans-synaptic tracing strategy, morphological results showed that urination-related PMC-projecting VLPAG neurons received dense inputs from multiple urination-related higher brain areas, such as the medial preoptic area, medial prefrontal cortex, and lateral hypothalamus. Thus, our findings reveal a novel insight into the VLPAG for control of bladder function and provide a potential therapeutic midbrain node for neurogenic bladder dysfunction.
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