The trehalose pathway in maize: conservation and gene regulation in response to the diurnal cycle and extended darkness.
The trehalose pathway in maize: conservation and gene regulation in response to the diurnal cycle and extended darkness.
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DOI：
10.1093/jxb/eru335
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发表时间：
2014-11
影响因子：
6.9
通讯作者：
Lagrimini LM
中科院分区：
文献类型：
--
作者：
Henry C;Bledsoe SW;Siekman A;Kollman A;Waters BM;Feil R;Stitt M;Lagrimini LM
延长黑暗诱导糖和海藻糖途径基因表达的瞬时增加。工厂中的能源资源在不断变化的环境中进行管理，例如在白天/夜晚周期中发生的变化。遮荫是一种环境破坏，降低光合作用，损害能量状态，影响作物生产力。海藻糖途径在维持能量平衡中起着重要但尚未明确的作用。在这里，我们的特点是玉米海藻糖途径基因和破译的昼夜周期和破坏的白天/黑夜周期的海藻糖途径基因表达和糖代谢的影响。玉米基因组编码14个海藻糖-6-磷酸合酶（TPS）基因、11个海藻糖-6-磷酸磷酸酶（TPP）基因和1个海藻糖酶基因。这些基因中的大多数的转录丰度受到日/夜周期和延长的黑暗胁迫的影响，蔗糖、己糖、淀粉和海藻糖-6-磷酸（T6P）水平也受到影响。延长黑暗后，T6P水平与II类TPS和蔗糖非发酵相关蛋白激酶1（SnRK1）靶基因表达呈负相关。最重要的是，T6P不再跟踪延长黑暗后的蔗糖水平。这些结果表明：（i）玉米中海藻糖途径的保守性;（ii）蔗糖、己糖、淀粉、T6P和TPS/TPP转录物响应昼夜循环;和（iii）延长的黑暗破坏T6P和蔗糖/己糖池之间的相关性并影响SnRK1靶基因表达。提出了一个海藻糖途径在玉米幼苗蔗糖和能量状态感知中的作用模型。
Extended darkness induces a transient increase in sugars and trehalose pathway gene expression. Energy resources in plants are managed in continuously changing environments, such as changes occurring during the day/night cycle. Shading is an environmental disruption that decreases photosynthesis, compromises energy status, and impacts on crop productivity. The trehalose pathway plays a central but not well-defined role in maintaining energy balance. Here, we characterized the maize trehalose pathway genes and deciphered the impacts of the diurnal cycle and disruption of the day/night cycle on trehalose pathway gene expression and sugar metabolism. The maize genome encodes 14 trehalose-6-phosphate synthase (TPS) genes, 11 trehalose-6-phosphate phosphatase (TPP) genes, and one trehalase gene. Transcript abundance of most of these genes was impacted by the day/night cycle and extended dark stress, as were sucrose, hexose sugars, starch, and trehalose-6-phosphate (T6P) levels. After extended darkness, T6P levels inversely followed class II TPS and sucrose non-fermenting-related protein kinase 1 (SnRK1) target gene expression. Most significantly, T6P no longer tracked sucrose levels after extended darkness. These results showed: (i) conservation of the trehalose pathway in maize; (ii) that sucrose, hexose, starch, T6P, and TPS/TPP transcripts respond to the diurnal cycle; and(iii) that extended darkness disrupts the correlation between T6P and sucrose/hexose pools and affects SnRK1 target gene expression. A model for the role of the trehalose pathway in sensing of sucrose and energy status in maize seedlings is proposed.
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