Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model.
Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model.
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DOI：
10.1016/j.bbrep.2021.101160
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发表时间：
2021-12
影响因子：
2.7
通讯作者：
Tanemura K
中科院分区：
文献类型：
--
作者：
Sakai K;Hiradate Y;Hara K;Tanemura K
睾丸毒性是癌症化疗和药物开发中的一个主要问题，因为它可能导致不孕症;然而，这种不良反应没有有效的生物标志物。为了鉴定新的生物标志物，我们研究了小的非编码RNA（sncRNA）在阿霉素（DXR）诱导的睾丸毒性小鼠模型中的表达。首先，我们对DXR处理或对照小鼠的精子进行了小RNA测序分析，并观察到许多基因组衍生序列的差异表达。然后，我们对这些序列进行实时RT-PCR验证，发现由一个引物dxRN_3检测到的sncRNA显示出与RNA-seq实验中所见相似的差异表达。这些发现表明，存在于精子中的sncRNA有可能作为临床上可接受的睾丸毒性生物标志物。睾丸毒性是癌症化疗和药物开发中的主要关注点。缺乏有效的生物标志物来评估睾丸毒性。对来自多柔比星处理的小鼠的精子进行小RNA-seq分析。差异RNA表达分析鉴定了小的非编码RNA。精子中的小分子非编码RNA可能是睾丸毒性的有用生物标志物。
Testicular toxicity is a major concern in cancer chemotherapy and drug development as it can result in infertility; however, there are no effective biomarkers for this adverse effect. To identify new biomarkers, we investigated the expression of small non-coding RNAs (sncRNAs) in a mouse model of doxorubicin (DXR)-induced testicular toxicity. First, we performed small RNA-seq analysis of sperm from DXR-treated or control mice and observed differential expression of many genome-derived sequences. We then performed real-time RT-PCR validation of these sequences and discovered that sncRNA detected by one primers, dxRN_3, showed similar differential expression as that seen in the RNA-seq experiment. These findings suggest that the sncRNAs present in sperm have potential as clinically acceptable biomarkers for testicular toxicity. Testicular toxicity is a major concern in cancer chemotherapy and drug development. There is a lack of effective biomarkers to assess testicular toxicity. Small RNA-seq analysis was performed on sperm from doxorubicin-treated mice. Differential RNA expression analyses identified a small non-coding RNA. Small non-coding RNAs in sperm may be useful biomarkers for testicular toxicity.
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