MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children.
MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children.
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腺病毒感染肺炎儿童全血 MicroRNA 表达谱发生改变
DOI：
10.1155/2018/2320640
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发表时间：
2018
影响因子：
4.6
通讯作者：
Lu G
中科院分区：
文献类型：
--
作者：
Huang F;Zhang J;Yang D;Zhang Y;Huang J;Yuan Y;Li X;Lu G
人类腺病毒（Adv）感染是导致大多数婴幼儿社区获得性肺炎的原因，每年都会导致大量儿童发病率和死亡率。MicroRNAs （miRNAs）与病毒复制和宿主免疫反应相关。了解miRNA表达谱将有助于了解miRNA在调节宿主对腺病毒感染反应中的作用，并可能提高腺病毒感染肺炎的诊断。本研究采用小RNA深度测序方法对腺病毒感染肺炎患儿和健康对照组全血中提取的总RNA进行分析。腺病毒感染儿童全血microRNAs表达谱发生改变，且差异明显。在腺病毒感染的儿童中发现了前3位上调的miRNA (hsa-miR-127-3p， hsa-miR-493-5p和hsa-miR-409-3p)，并提供了感染和健康个体之间的明确区分。通过qRT-PCR预测和验证潜在宿主靶基因，研究microrna对宿主基因的影响。大多数靶基因参与MAPK信号通路和先天免疫应答。这些高度上调的microrna可能在Adv发病机制中发挥关键作用，是腺病毒感染肺炎的潜在生物标志物。
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing. Expression profiles of whole blood microRNAs were altered and distinctly different in adenovirus-infected children. The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. Potential host target genes were predicated and validated by qRT-PCR to study the impact of microRNAs on the host genes. Most of the target genes were involved in the MAPK signaling pathway and innate immune response. These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia.
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影响因子：
12.8
作者：
Onnis, A.;Navari, M.;Antonicelli, G.;Morettini, F.;Mannucci, S.;De Falco, G.;Vigorito, E.;Leoncini, L.
通讯作者：
Leoncini, L.
影响因子：
168.9
作者：
Bhutta, Zulfiqar A.;Das, Jai K.;Black, Robert E.
通讯作者：
Black, Robert E.
DOI：
10.1111/tmi.12368
发表时间：
2014-11
期刊：
Tropical medicine & international health : TM & IH
影响因子：
--
作者：
Agweyu A;Kibore M;Digolo L;Kosgei C;Maina V;Mugane S;Muma S;Wachira J;Waiyego M;Maleche-Obimbo E
通讯作者：
Maleche-Obimbo E
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影响因子：
4.3
作者：
Kang, Wenyan;Shang, Lingling;Ge, Shaohua
通讯作者：
Ge, Shaohua
影响因子：
3.8
作者：
Simpson, LJ;Sale, JE
通讯作者：
Sale, JE