CRISPR/Cas9-Mediated Mutagenesis of Carotenoid Cleavage Dioxygenase 8 (CCD8) in Tobacco Affects Shoot and Root Architecture.
CRISPR/Cas9-Mediated Mutagenesis of Carotenoid Cleavage Dioxygenase 8 (CCD8) in Tobacco Affects Shoot and Root Architecture.
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烟草中 CRISPR/Cas9 介导的类胡萝卜素裂解双加氧酶 8 (CCD8) 诱变影响芽和根结构
DOI：
10.3390/ijms19041062
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发表时间：
2018-04-02
影响因子：
5.6
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Gao J;Zhang T;Xu B;Jia L;Xiao B;Liu H;Liu L;Yan H;Xia Q
植物内酯(SLS)是一类调节植物结构的植物激素。类胡萝卜素裂解双加氧酶(CCD)基因参与了SLS的生物合成，并在许多植物中被鉴定和鉴定。然而，烟草中的ccd基因的功能仍然知之甚少。本研究从烟草中克隆了两个紧密相关的基因NtCCD8A和NtCCD8B。这两个NtCCD8基因是番茄类胡萝卜素裂解双加氧酶8(SlCCD8)基因的同源基因。NtCCD8A和NtCCD8B主要在烟草根中表达，但在所有植物组织中都有低水平的表达，并且它们的转录水平随着磷限制的增加而显著增加。利用CRISPR/Cas9系统将NtCCD8A和NtCCD8B突变导入烟草，获得了含有这两个突变等位基因的转基因烟草品系。Ntccd8a和ntccd8b突变等位基因分别通过三个核苷酸的缺失和一个核苷酸的插入而失活，这两者都导致了过早终止密码子的产生。与野生型相比，ntccd8突变体增加了枝条分枝，降低了株高，增加了叶片数和节数，降低了植株总生物量，但根冠比没有变化。此外，突变体的主根比野生型更短，侧根更多。这些结果表明，NtCCD8基因对烟草植株结构的改变是重要的。
Strigolactones (SLs) are a class of phytohormones that regulate plant architecture. Carotenoid cleavage dioxygenase (CCD) genes are involved in the biosynthesis of SLs and are identified and characterized in many plants. However, the function of CCD genes in tobacco remains poorly understood. In this study, two closely related genes NtCCD8A and NtCCD8B were cloned from tobacco (Nicotiana tabacum L.). The two NtCCD8 genes are orthologues of the tomato (Solanum lycopersicum) carotenoid cleavage dioxygenase 8 (SlCCD8) gene. NtCCD8A and NtCCD8B were primarily expressed in tobacco roots, but low expression levels of these genes were detected in all plant tissues, and their transcript levels significantly increased in response to phosphate limitation. NtCCD8A and NtCCD8B mutations were introduced into tobacco using the CRISPR/Cas9 system and transgenic tobacco lines for both ntccd8 mutant alleles were identified. The ntccd8a and ntccd8b mutant alleles were inactivated by a deletion of three nucleotides and insertion of one nucleotide, respectively, both of which led to the production of premature stop codons. The ntccd8 mutants had increased shoot branching, reduced plant height, increased number of leaves and nodes, and reduced total plant biomass compared to wild-type plants; however, the root-to-shoot ratio was unchanged. In addition, mutant lines had shorter primary roots and more of lateral roots than wild type. These results suggest that NtCCD8 genes are important for changes in tobacco plant architecture.
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影响因子：
4.2
作者：
Akiyama, Kohki;Hayashi, Hideo
通讯作者：
Hayashi, Hideo
影响因子：
9.2
作者：
Booker, J;Auldridge, M;Leyser, O
通讯作者：
Leyser, O
影响因子：
7.4
作者：
Ferguson, Brett J.;Beveridge, Christine A.
通讯作者：
Beveridge, Christine A.
影响因子：
9.8
作者：
Besserer A;Puech-Pagès V;Kiefer P;Gomez-Roldan V;Jauneau A;Roy S;Portais JC;Roux C;Bécard G;Séjalon-Delmas N
通讯作者：
Séjalon-Delmas N
影响因子：
7.4
作者：
Dun, Elizabeth A.;de Saint Germain, Alexandre;Beveridge, Christine A.
通讯作者：
Beveridge, Christine A.