Impact of diesel exhaust particles on infections with Mycobacterium bovis BCG in in vitro human macrophages and an in vivo Galleria mellonella model.
Impact of diesel exhaust particles on infections with Mycobacterium bovis BCG in in vitro human macrophages and an in vivo Galleria mellonella model.
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DOI：
10.1016/j.envpol.2023.122597
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发表时间：
2024-01-15
影响因子：
8.9
通讯作者：
Dominguez, Jose
中科院分区：
文献类型：
--
作者：
Rodriguez-Fernandez, Pablo;Romero-Andrada, Iris;Molina-Moya, Barbara;Latorre, Irene;Lacoma, Alicia;Prat-Aymerich, Cristina;Tabernero, Lydia;Dominguez, Jose
有强烈的迹象表明肺结核（TB）和空气质量之间存在联系。柴油机废气是污染的主要贡献者之一，据报道，它能够改变肺部感染的易感性。在这项研究中，我们暴露THP-1人巨噬细胞和牛分枝杆菌卡介苗柴油机排气颗粒（DEPs）。THP-1细胞在3和6天时具有较高的细胞毒活性和凋亡激活作用，但对M.牛卡介苗感染的THP-1细胞暴露于非细胞毒性DEP浓度显示出有限的能力，吞噬乳胶珠。然而，M。牛BCG感染巨噬细胞并不导致细菌负荷的增加，但它确实导致从细胞外培养基中回收的细菌的增加，这表明M.牛卡介苗我们还观察到DEP暴露限制了细胞因子的产生。使用大蜡螟感染模型，我们观察到暴露于低水平DEP的幼虫在感染M. bovis BCG，并且在感染4天后具有更高的内部细菌负荷。解开空气污染与人体抗分枝杆菌免疫力受损之间的联系至关重要，因为在结核病发病率极高的地区，污染正在迅速增加。柴油机尾气颗粒物（DEPs）对人巨噬细胞具有强烈的毒性，能强烈地内化并增加巨噬细胞的凋亡，从而限制了巨噬细胞吞噬乳胶粒的能力。人巨噬细胞DEPs内的牛BCG损害细胞因子TNF-α、IL-8、IL-1β和GM-CSF的产生。DEPs降低了M. bovis BCG感染G.在无毒浓度下的大蜡螟。
There are strong suggestions for a link between pulmonary tuberculosis (TB) and air quality. Diesel exhaust is one of the main contributors to pollution and it is reported to be able to modify susceptibility to lung infections. In this study we exposed THP-1 human macrophages and Mycobacterium bovis BCG to diesel exhaust particles (DEPs). High cytotoxicity and activation of apoptosis was found in THP-1 cells at 3 and 6 days, but no effect was found on the growth of M. bovis BCG. Infection of THP-1 cells exposed to a non-cytotoxic DEP concentration showed a limited capacity to engulf latex beads. However, M. bovis BCG infection of macrophages did not result in an increase in the bacterial burden, but it did result in an increase in the bacteria recovered from the extracellular media, suggesting a poor contention of M. bovis BCG. We also observed that DEP exposure limited the production of cytokines. Using the Galleria mellonella model of infection, we observed that larvae exposed to low levels of DEPs were less able to survive after infection with M. bovis BCG and had a higher internal bacterial load after 4 days of infection. Unraveling the links between air pollution and impairment of human antimycobacterial immunity is vital, because pollution is rapidly increasing in areas where TB incidence is extremely high. Diesel exhaust particles (DEPs) are highly toxic to human macrophages, avidly internalized and increase the apoptosis DEPs limit the ability of macrophages to engulf latex beads DEPs difficult the contention of M. bovis BCG inside human macrophages DEPs impairs the production of cytokines TNF-α, IL-8, IL-1β and GM-CSF. DEPs decrease the survival in M. bovis BCG infected G. mellonella at non-toxic concentrations.
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影响因子：
--
作者：
Bonay, M;Chambellan, A;Soler, P
通讯作者：
Soler, P
影响因子：
3.9
作者：
Lin, Yi-Jun;Lin, Hsing-Chieh;Liao, Chung-Min
通讯作者：
Liao, Chung-Min
影响因子：
10.4
作者：
Blount RJ;Pascopella L;Catanzaro DG;Barry PM;English PB;Segal MR;Flood J;Meltzer D;Jones B;Balmes J;Nahid P
通讯作者：
Nahid P
影响因子：
2.7
作者：
Dumax-Vorzet AF;Tate M;Walmsley R;Elder RH;Povey AC
通讯作者：
Povey AC
影响因子：
3.7
作者：
Gomez, Andromeda-Celeste;Rodriguez-Fernandez, Pablo;Dominguez, Jose
通讯作者：
Dominguez, Jose