Localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibers.
Localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibers.
复制标题
DOI：
10.1016/j.autneu.2009.07.010
复制
发表时间：
2009-12-03
影响因子：
2.7
通讯作者：
Kreulen, David
中科院分区：
文献类型：
--
作者：
Cao, Xian;Demel, Stacie L.;Quinn, Mark T.;Galligan, James J.;Kreulen, David
关键词：
先前报道称，在 DOCA 盐高血压期间，腹腔神经节 (CG) 的超氧阴离子 (O2−•) 产量增加，这可能是通过激活还原型烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 氧化酶实现的。这表明神经元 NADPH 氧化酶在自主神经血管控制中发挥作用。然而，NADPH氧化酶在外周神经元中的表达和定位尚不完全清楚。本研究的目的是检查 NADPH 氧化酶在交感神经节和感觉神经节神经元以及血管周围神经纤维中的亚细胞定位。在大鼠 CG 中，p22phox 和神经肽 Y (NPY) 共定位于所有神经元中。 P22phox 还定位于含有降钙素基因相关肽 (CGRP) 的背根神经节 (DRG) 神经元。在肠系膜动脉中，p22phox 和 p47phox 与血管周围神经末梢的 NPY 或 CGRP 共定位。在培养的 CG 神经元和神经生长因子 (NGF) 分化的 PC12 细胞中也发现了类似的 p22phox 和 p47phox 神经末梢染色模式。这些数据证明了 NADPH 氧化酶在血管周围神经纤维中的先前未表征的定位。神经纤维中神经递质处理位点附近存在 O2−• – 生成酶，这表明在周围神经血管控制中可能存在新颖的氧化还原介导机制。
Superoxide anion (O2−•) production was previously reported to be increased in celiac ganglia (CG) during DOCA-salt hypertension, possibly via activation of the reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase. This suggested a role for neuronal NADPH oxidase in autonomic neurovascular control. However, the expression and localization of NADPH oxidase in the peripheral neurons is not fully known. The purpose of this study was to examine the subcellular localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibers. In rat CG, p22phox and neuropeptide Y (NPY) were colocalized in all neurons. P22phox was also localized to dorsal root ganglia (DRG) neurons that contain calcitonin gene related peptide (CGRP). In mesenteric arteries, p22phox and p47phox were colocalized with NPY or CGRP in perivascular nerve terminals. A similar pattern of nerve terminal staining of p22phox and p47phox was also found in cultured CG neurons and nerve growth factor (NGF)-differentiated PC12 cells. These data demonstrate a previously uncharacterized localization of NADPH oxidase in perivascular nerve fibers. The presence of a O2−• – generating enzyme in close vicinity to the sites of neurotransmitter handling in the nerve fibers suggests the possibility of novel redox-mediated mechanisms in peripheral neurovascular control.
登录
查看更多内容
影响因子：
8.3
作者：
Beswick, RA;Dorrance, AM;Webb, RC
通讯作者：
Webb, RC
影响因子：
20.1
作者：
GRIENDLING, KK;MINIERI, CA;ALEXANDER, RW
通讯作者：
ALEXANDER, RW
DOI：
10.1152/ajpheart.1999.276.4.h1263
发表时间：
1999-04-01
影响因子：
4.8
作者：
Browning, KN;Zheng, ZL;Travagli, RA
通讯作者：
Travagli, RA
影响因子：
8.3
作者：
Cao, Xian;Dai, Xiaoling;Kreulen, David L.
通讯作者：
Kreulen, David L.
影响因子：
2.9
作者：
Günes, A;Ceylan, A;Karasu, Ç
通讯作者：
Karasu, Ç