Fluorogenic 'click-on' dendrimer reporter for rapid profiling of cell proliferation.
Fluorogenic 'click-on' dendrimer reporter for rapid profiling of cell proliferation.
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用于快速分析细胞增殖的荧光“点击式”树枝状聚合物报告基因。
DOI：
10.1016/j.bmcl.2013.01.061
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发表时间：
2013
影响因子：
2.7
通讯作者：
Zong,Hong
中科院分区：
文献类型：
--
作者：
Goonewardena,SaschaN;Leroueil,PascaleR;Gemborys,Colleen;Tahiliani,Pawan;Emery,Sarah;BakerJr,JamesR;Zong,Hong
The application of small molecule fluorescent reporters to monitor biological systems is limited by their poor water solubility and background fluorescence of these reporters. Herein, we describe the synthesis and testing of a fluorogenic ‘click’ dendrimer reporter to monitor cellular processes. The reporter system consists of a polyamidoamine (PAMAM) dendrimer conjugated with 3-azido-7-hydroxy coumarin. After the copper(I)-catalyzed azide–alkyne cycloaddition reaction (‘click’ reaction) with alkyne-derivatized target molecules, the natively non-fluorescent construct has a strong enhancement in fluorescence. This fluorogenic dendrimer reporter can be used to efficiently monitor biological processes and the specificity afforded by the ‘click’ reaction greatly reduces background noise and enhances assay flexibility. We used this fluorogenic dendrimer reporter to monitor incorporation of 5-ethynyl-2′-deoxyuridine (EdU) into newly synthesized DNA, as a surrogate marker of cellular proliferation. We anticipate that this new class of fluorogenic reporter can be used to monitor a wide array of molecules and lends itself to high-throughput profiling of biological systems.
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影响因子：
46.2
作者：
Jewett JC;Bertozzi CR
通讯作者：
Bertozzi CR
影响因子：
56.9
作者：
Griffin, BA;Adams, SR;Tsien, RY
通讯作者：
Tsien, RY
影响因子：
2.9
作者：
Zhang, ZW;Smith, BAC;Schultz, PG
通讯作者：
Schultz, PG
影响因子：
15
作者：
Yamakoshi, Hiroyuki;Dodo, Kosuke;Sodeoka, Mikiko
通讯作者：
Sodeoka, Mikiko
DOI：
10.1073/pnas.0403413101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Kho, Y;Kim, SC;Zhao, YM
通讯作者：
Zhao, YM