Upregulation of CD94 on CD8+T cells in anterior chamber-associated immune deviation.
Upregulation of CD94 on CD8+T cells in anterior chamber-associated immune deviation.
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前房相关免疫偏差中 CD8 T 细胞上 CD94 的上调
DOI：
10.1186/1471-2172-9-53
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发表时间：
2008-09-25
期刊：
影响因子：
3
通讯作者：
Kijlstra, Aize
中科院分区：
文献类型：
--
作者：
He, Hao;Yang, Peizeng;Jiang, Liqiong;Zhang, Junfeng;Zhao, Changlin;Chen, Lina;Lin, Xiaomin;Zhou, Hongyan;Kijlstra, Aize
背景CD 8+调节性T细胞（Treg）被认为参与了眼内免疫耐受模型，即前房相关免疫偏离（ACAID）。ACAID中CD 8 +Treg的表型和特征仍然知之甚少。近年来的研究表明，CD 94-Qa-1系统参与了ACAID小鼠CD 8 +Treg的诱导，但CD 8 + CD 94 +T细胞的功能和特性尚不清楚。流式细胞术分析显示，极少数的CD 8 + CD 94 +T细胞表达颗粒酶B、穿孔素和Foxp 3。从ACAID小鼠脾脏磁性分离的CD 8 + CD 94 +T细胞，而不是CD 8 + CD 94-T细胞，产生大量的TGF-β 1，并在体外表现出抑制活性。结论ACAID小鼠的CD 8 + CD 94 + T细胞具有明显的免疫抑制作用，其抑制作用与TGF-β 1的表达增强有关，提示CD 8 +Treg主要分布于CD 94 +T细胞亚群。
BackgroundCD8+regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The phenotype and characteristics of CD8+Treg in ACAID remain only poorly understood. Recent studies have reported that the CD94-Qa-1 system is implicated in the induction of ACAID CD8+Treg, but the functions and characteristics of CD8+CD94+T cells remain unclear.ResultsBoth mRNA and protein of CD94 and NKG2A were markedly up-regulated on splenic CD8+T cells of ACAID mice compared with controls. Flow cytometric analysis showed that very few CD8+CD94+T cells express granzyme B, perforin and Foxp3. CD8+CD94+T cells, but not CD8+CD94-T cells, magnetically isolated from the spleens of ACAID mice, produced large amounts of TGF-beta1 and exhibited suppressive activity in vitro. Neutralization of TGF-beta1 caused reversal of suppression mediated by CD8+CD94+T cells.ConclusionCD8+CD94+T cells from ACAID mice exhibited suppressive activity in association with enhanced expression of TGF-beta1, suggesting that CD8+Treg are mainly distributed in CD94+T cell subpopulations.
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