Gallotannin suppresses calcium oxalate crystal binding and oxalate-induced oxidative stress in renal epithelial cells.
Gallotannin suppresses calcium oxalate crystal binding and oxalate-induced oxidative stress in renal epithelial cells.
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Gallotannin抑制草酸钙晶体结合和草酸盐诱导的肾上皮细胞中的氧化应激。
DOI：
10.1248/bpb.35.539
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发表时间：
2012
影响因子：
2
通讯作者：
Kim SH
中科院分区：
文献类型：
--
作者：
Lee HJ;Jeong SJ;Park MN;Linnes M;Han HJ;Kim JH;Lieske JC;Kim SH
一水草酸钙（COM）晶体与增殖和迁移的肾内皮细胞表面结合，这可能是肾结石形成的关键事件。草酸诱导的预氧化应激可进一步促进晶体附着细胞。天然产品，包括在绿色茶中发现的没食子单宁，已经被研究作为潜在的新治疗方法，以防止晶体滞留和肾结石形成。在无毒浓度下，没食子单宁显著抑制COM晶体生长和与MDCK I肾上皮细胞的结合，并且在伤口愈合测定中也延迟肾细胞迁移。逆转录聚合酶链反应（RT-PCR）分析显示，没食子单宁显着减弱柠檬酸诱导的单核细胞趋化蛋白1（MCP-1），骨桥蛋白（OPN），烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶亚基p22 phox和p47 phox在人原代肾上皮细胞（HRCs）的mRNA表达。没食子单宁也减少HRC生产的活性氧（ROS）和丙二醛（MDA），以及增强抗氧化酶超氧化物歧化酶（SOD）的活性响应草酸。总之，我们的研究结果表明，没食子单宁可以通过对肾上皮细胞的直接作用，包括抑制COM结合和MCP-1和OPN表达，沿着增强抗氧化活性，来预防肾结石。
Calcium oxalate monohydrate (COM) crystals bind avidly to the surface of proliferating and migrating renal endothelial cells, perhaps a key event in kidney stone formation. Oxalate-induced pre-oxidative stress can further promote crystal attachment cells. Natural products including gallotannins found in green teas have been studied as potentially novel treatments to prevent crystal retention and kidney stone formation. Gallotannin significantly inhibited COM crystal growth and binding to MDCK I renal epithelial cells at non-toxic concentrations and also delayed renal cell migration in a wound healing assay. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that gallotannin significantly attenuated oxalate-induced mRNA expression of monocyte chemoattractant protein 1 (MCP-1), osteopontin (OPN), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit p22phox and p47phox in human primary renal epithelial cells (HRCs). Gallotannin also reduced HRC production of reactive oxygen species (ROS) and malondialdehyde (MDA) as well as enhanced antioxidant enzyme superoxide dismutase (SOD) activity in response to oxalate. Taken together, our findings suggest that gallotannin can contribute to nephrolithiasis prevention via direct effects on renal epithelial cells including suppression of COM binding and MCP-1 and OPN expression, along with augmenting antioxidant activity.
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影响因子：
4.5
作者：
Boonla, Chanchai;Hunapathed, Chanutra;Tosukhowong, Piyaratana
通讯作者：
Tosukhowong, Piyaratana
影响因子：
6.6
作者：
Thamilselvan, S;Byer, KJ;Khan, SR
通讯作者：
Khan, SR
1,2,3,4,6-PENTA-O-GALLOYL-BETA-D-葡萄糖可在高氧大鼠模型中降低肾脏结晶和氧化应激。
影响因子：
19.6
作者：
Lee HJ;Jeong SJ;Lee HJ;Lee EO;Bae H;Lieske JC;Kim SH
通讯作者：
Kim SH
影响因子：
6.1
作者：
Dörrenhaus, A;Müller, JIF;Föllmann, W
通讯作者：
Föllmann, W
DOI：
10.1152/ajprenal.1995.268.4.f604
发表时间：
1995-04-01
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY
影响因子：
--
作者：
LIESKE, JC;LEONARD, R;TOBACK, FG
通讯作者：
TOBACK, FG