Thymoquinone, a bioactive component of Nigella sativa Linn seeds or traditional spice, attenuates acute hepatic failure and blocks apoptosis via the MAPK signaling pathway in mice
Thymoquinone, a bioactive component of Nigella sativa Linn seeds or traditional spice, attenuates acute hepatic failure and blocks apoptosis via the MAPK signaling pathway in mice
复制标题
百里醌是黑种草种子或传统香料的生物活性成分，可通过 MAPK 信号通路减轻小鼠急性肝衰竭并阻止细胞凋亡
DOI：
10.1039/c4ra15065a
复制
发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Nan Ji-Xing
中科院分区：
文献类型：
--
作者：
Yang Yong;Bai Ting;Sun Peng;Lian Li-Hua;Yao You-Li;Zheng Hui-Xing;Li Xin;Li Jin-Bin;Wu Yan-Ling;Nan Ji-Xing
胸腺醌（Thymoquinone，TQ）是从黑种草子（Nigella sativa Linn）的黑孜然籽中提取的具有生物活性的天然产物，是一种广泛应用的香料或草药。在注射D-GalN（700 mg kg −1）/LPS（10 μg kg − 1）之前，对小鼠灌胃TQ（5或20 mg kg − 1）12 h和1 h，然后在用D-GalN/LPS处理后8 h处死。TQ预处理可降低D-GalN/LPS诱导的小鼠死亡率，逆转肝损伤。TQ减弱D-GalN/LPS诱导的肝细胞凋亡，这通过抑制caspase活化、PARP裂解和Bax/Bcl-2比率来证实。重要的是，TQ减弱了D-GalN/LPS介导的JNK，ERK和p38的磷酸化。此外，TQ抑制促炎细胞因子的产生。这些结果表明，TQ可以通过抑制caspase激活来调节D-GalN/LPS介导的急性肝衰竭，这与线粒体凋亡途径和MAPK信号通路一致。
Thymoquinone (TQ), a bioactive natural product obtained from the black cumin seeds of Nigella sativa Linn, is a widely used spice or herb. The present study investigated the hepatoprotective effect of TQ on acute hepatic failure induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice. The mice were intragastrically administrated TQ (5 or 20 mg kg−1) for 12 h and 1 h prior to D-GalN (700 mg kg−1)/LPS (10 μg kg−1) injections and then sacrificed 8 h after treatment with D-GalN/LPS. TQ pretreatment reduced the mortality induced by D-GalN/LPS and reversed liver damage. TQ attenuated D-GalN/LPS-induced hepatocyte apoptosis, which was confirmed by suppressing caspase activation, PARP cleavage and the Bax/Bcl-2 ratio. Importantly, TQ attenuated the D-GalN/LPS-mediated phosphorylation of JNK, ERK and p38. Furthermore, TQ suppressed the production of proinflammatory cytokines. These findings suggested that TQ could modulate D-GalN/LPS-mediated acute hepatic failure by inhibiting caspase activation, consistent with the mitochondrial pathway of apoptosis and the MAPK signaling pathway.
登录
查看更多内容
百里醌通过激活的肝星状细胞中的 PI3K 和 TLR4 信号通路减轻肝纤维化
影响因子：
5.6
作者：
Bai, Ting;Lian, Li-Hua;Nan, Ji-Xing
通讯作者：
Nan, Ji-Xing
影响因子：
--
作者：
Liu R;Li JZ;Song JK;Sun JL;Li YJ;Zhou SB;Zhang TT;Du GH
通讯作者：
Du GH
Pim-3 可预防 D-半乳糖胺 (D-GalN) 致敏大鼠的肝衰竭
DOI：
10.1111/j.1365-2362.2009.02235.x
发表时间：
2010-02-01
影响因子：
5.5
作者：
Liu, L. -M.;Zhang, J. -X.;Luo, J.
通讯作者：
Luo, J.
影响因子：
4.6
作者：
Kang, Seok Yong;Jung, Hyo Won;Park, Yong-Ki
通讯作者：
Park, Yong-Ki
影响因子：
7.9
作者：
Ghazwani, Mohammed;Zhang, Yifei;Li, Song
通讯作者：
Li, Song