Identification of the binding site of two monoclonal antibodies to human protamine.
Identification of the binding site of two monoclonal antibodies to human protamine.
复制标题
两种单克隆抗体与人鱼精蛋白的结合位点的鉴定。
DOI：
10.1016/0161-5890(93)90436-f
复制
发表时间：
1993
影响因子：
3.6
通讯作者：
R. Balhorn
中科院分区：
文献类型：
--
作者：
L. Stanker;A. Wyrobek;C. McKeown;R. Balhorn
我们之前开发了许多与鱼精蛋白结合的单克隆抗体 (Mab)。其中一种抗体 Hup1N 与人鱼精蛋白 1 结合，但不与鱼精蛋白 2 结合。相比之下，Mab HupA 同时结合鱼精蛋白 1 和鱼精蛋白 2。观察到这两种 Mab 的表位重叠，并且定位于鱼精蛋白 1 的进化保守氨基末端区域。此分配基于 ob 抗体与鱼精蛋白序列已知的不同物种的鱼精蛋白结合，以及抗体分析与合成肽和具有特定氨基酸取代的合成肽结合。
We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to the evolutionarily conservative ammo-terminal region of protamine 1. This assignment is based ob antibody binding to protamine from different species in which the protamine sequence is known, as well as analysis of antibody binding to synthetic peptides and synthetic peptides with specific amino acid substitutions.
影响因子：
2.9
作者：
K. Kleene;R. Distel;N. Hecht
通讯作者：
K. Kleene;R. Distel;N. Hecht
影响因子：
3.7
作者：
BUNICK, D;BALHORN, R;HECHT, NB
通讯作者：
HECHT, NB