A single-cell transcriptomic atlas tracking the neural basis of division of labour in an ant superorganism.
A single-cell transcriptomic atlas tracking the neural basis of division of labour in an ant superorganism.
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单细胞转录组图谱追踪蚂蚁超有机体分工的神经基础
DOI：
10.1038/s41559-022-01784-1
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发表时间：
2022-08
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
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作者：
种姓之间具有永久分工和性别角色高度不同的蚁群已被概念化为超有机体，但介导种姓/性别特异性行为专业化的细胞和分子机制仍然模糊。在这里，我们通过获得 206,367 个单核转录组来表征法老王蚁后、雌性（处女蚁后）、工蚁和雄性的脑细胞库。与果蝇相反，蚂蚁的蘑菇体凯尼恩细胞丰富，并且表现出高度的多样性，大多数亚型在工蚁大脑（进化衍生的种姓）中丰富。雄性大脑与工蚁大脑一样特化，但细胞组成趋势相反，所有视叶神经元亚型的丰度更高，而雌性和蚁后大脑的组成仍然普遍化，让人想起孤独的祖先。从处女雌虫到受精蜂王的角色分化引起大约 35% 的细胞类型丰度变化，表明在这一转变过程中存在活跃的神经发生和/或程序性细胞死亡。我们还发现了授精诱导的细胞变化可能与生殖种姓的寿命和繁殖力相关，包括鞘神经胶质细胞和表达多巴胺调节的 Dh31 的神经元群体的增加。我们得出的结论是，永久的种姓分化和极端的性别分化引起了蚂蚁神经回路的重大变化。
Ant colonies with permanent division of labour between castes and highly distinct roles of the sexes have been conceptualized to be superorganisms, but the cellular and molecular mechanisms that mediate caste/sex-specific behavioural specialization have remained obscure. Here we characterized the brain cell repertoire of queens, gynes (virgin queens), workers and males ofMonomorium pharaonisby obtaining 206,367 single-nucleus transcriptomes. In contrast toDrosophila, the mushroom body Kenyon cells are abundant in ants and display a high diversity with most subtypes being enriched in worker brains, the evolutionarily derived caste. Male brains are as specialized as worker brains but with opposite trends in cell composition with higher abundances of all optic lobe neuronal subtypes, while the composition of gyne and queen brains remained generalized, reminiscent of solitary ancestors. Role differentiation from virgin gynes to inseminated queens induces abundance changes in roughly 35% of cell types, indicating active neurogenesis and/or programmed cell death during this transition. We also identified insemination-induced cell changes probably associated with the longevity and fecundity of the reproductive caste, including increases of ensheathing glia and a population of dopamine-regulatedDh31-expressing neurons. We conclude that permanent caste differentiation and extreme sex-differentiation induced major changes in the neural circuitry of ants.
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
DOI：
10.1126/science.aar5723
发表时间：
2018-07-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chandra V;Fetter-Pruneda I;Oxley PR;Ritger AL;McKenzie SK;Libbrecht R;Kronauer DJC
通讯作者：
Kronauer DJC
影响因子：
1.5
作者：
Allard, D.;Borgesen, L.;Gobin, B.
通讯作者：
Gobin, B.
影响因子：
3.6
作者：
Greiner, B;Ribi, WA;Warrant, EJ
通讯作者：
Warrant, EJ