Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection.
Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection.
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DOI：
10.1016/j.cccn.2005.06.023
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发表时间：
2006-01
期刊：
影响因子：
--
通讯作者：
Dunbar SA
中科院分区：
文献类型：
--
作者：
Dunbar SA
随着我们进入后基因组测序时代，并且开始筛选现在可用的大量遗传信息，对允许快速、成本有效、高通量检测特定核酸序列的技术的需求变得明显。允许在单个反应中同时检测多个核酸序列的多重技术可以大大减少与单个反应检测技术相关的时间、成本和劳动力。Luminex® xMAP™系统是一种基于多重微球的悬浮阵列平台，能够在单个反应容器中分析和报告多达100种不同的反应。该技术为高通量核酸检测提供了一个新的平台，并且正在越来越频繁地使用。本文就xMAP技术在单核苷酸多态性（SNP）基因分型、遗传性疾病筛查、基因表达谱分析、HLA DNA分型和微生物检测等方面的具体应用作一综述。这些研究证明了xMAP技术用于同时、快速、灵敏和特异性核酸检测的速度、效率和实用性，以及其满足分子实验室当前和未来高通量核酸检测要求的能力。
As we enter the post-genome sequencing era and begin to sift through the enormous amount of genetic information now available, the need for technologies that allow rapid, cost-effective, high-throughput detection of specific nucleic acid sequences becomes apparent. Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can greatly reduce the time, cost and labor associated with single reaction detection technologies. The Luminex® xMAP™ system is a multiplexed microsphere-based suspension array platform capable of analyzing and reporting up to 100 different reactions in a single reaction vessel. This technology provides a new platform for high-throughput nucleic acid detection and is being utilized with increasing frequency. Here we review specific applications of xMAP technology for nucleic acid detection in the areas of single nucleotide polymorphism (SNP) genotyping, genetic disease screening, gene expression profiling, HLA DNA typing and microbial detection. These studies demonstrate the speed, efficiency and utility of xMAP technology for simultaneous, rapid, sensitive and specific nucleic acid detection, and its capability to meet the current and future requirements of the molecular laboratory for high-throughput nucleic acid detection.
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