Synergistic effects of GhSOD1 and GhCAT1 overexpression in cotton chloroplasts on enhancing tolerance to methyl viologen and salt stresses.
Synergistic effects of GhSOD1 and GhCAT1 overexpression in cotton chloroplasts on enhancing tolerance to methyl viologen and salt stresses.
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GHSOD1和GHCAT1过表达在棉叶绿体中的协同作用对增强对甲基元素和盐胁迫的耐受性。
DOI：
10.1371/journal.pone.0054002
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tian Y
中科院分区：
文献类型：
--
作者：
Luo X;Wu J;Li Y;Nan Z;Guo X;Wang Y;Zhang A;Wang Z;Xia G;Tian Y
在植物中，铜锌超氧化物歧化酶（CuZnSOD, EC l.15.1.1）、抗坏血酸过氧化物酶（APX, EC 1.11.1.11）和过氧化氢酶（CAT, EC l.11.1.6）是重要的活性氧（ROS）清除剂，保护细胞免受伤害。在本研究中，我们从棉中分离到了三个同源基因（GhSOD1、GhAPX1和GhCAT1）。通过农杆菌转化将含有嵌合GhSOD1、GhAPX1或GhCAT1的过表达盒导入棉花植株，这些基因的过表达产物如预期的那样通过转运肽转运到叶绿体中。培育出过表达GhSOD1、GhAPX1、GhCAT1、GhSOD1和GhAPX1堆叠（SAT）以及GhSOD1和GhCAT1堆叠（SCT）的5种转基因棉花植株。温室分析表明，转基因植株对甲基紫病菌（MV）和盐的耐受性高于野生型植株。有趣的是，SCT植株在胁迫条件下没有受到损害。基于酶活性、电解质泄漏、叶绿素含量、光化学产量（Fv/Fm）和胁迫下生物量积累的分析，同时过表达GhSOD1和GhCAT1的SCT植株受益于两个基因的协同效应，对MV和盐胁迫表现出最高的耐受性，而同时过表达GhSOD1和GhAPX1的SAT植株则没有。此外，在叶绿体中过表达抗氧化酶的转基因植株比在细胞质中表达抗氧化酶的转基因植株对盐胁迫的耐受性更高，尽管总体酶活性几乎相同。因此，GhSOD1和GhCAT1在叶绿体中的协同作用为提高胁迫耐受性以避免产量损失提供了新的策略。
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous genes (GhSOD1, GhAPX1, and GhCAT1) from Gossypium hirsutum. Overexpressing cassettes containing chimeric GhSOD1, GhAPX1, or GhCAT1 were introduced into cotton plants by Agrobacterium transformation, and overexpressed products of these genes were transported into the chloroplasts by transit peptide, as expected. The five types of transgenic cotton plants that overexpressed GhSOD1, GhAPX1, GhCAT1, GhSOD1 and GhAPX1 stack (SAT), and GhSOD1 and GhCAT1 stack (SCT) were developed. Analyses in the greenhouse showed that the transgenic plants had higher tolerance to methyl viologen (MV) and salinity than WT plants. Interestingly, SCT plants suffered no damage under stress conditions. Based on analyses of enzyme activities, electrolyte leakage, chlorophyll content, photochemical yield (Fv/Fm), and biomass accumulation under stresses, the SCT plants that simultaneously overexpressed GhSOD1 and GhCAT1 appeared to benefit from synergistic effects of two genes and exhibited the highest tolerance to MV and salt stress among the transgenic lines, while the SAT plants simultaneously overexpressing GhSOD1 and GhAPX1 did not. In addition, transgenic plants overexpressing antioxidant enzymes in their chloroplasts had higher tolerance to salt stress than those expressing the genes in their cytoplasms, although overall enzyme activities were almost the same. Therefore, the synergistic effects of GhSOD1 and GhCAT1 in chloroplasts provide a new strategy for enhancing stress tolerance to avoid yield loss.
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影响因子：
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作者：
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