Transient ultrasound stimulation has lasting effects on neuronal excitability.
Transient ultrasound stimulation has lasting effects on neuronal excitability.
复制标题
DOI：
10.1016/j.brs.2021.01.003
复制
发表时间：
2021-03
影响因子：
7.7
通讯作者：
Whitcomb DJ
中科院分区：
文献类型：
--
作者：
Clennell B;Steward TGJ;Elley M;Shin E;Weston M;Drinkwater BW;Whitcomb DJ
经颅超声刺激可以剧烈调节大脑活动，但对神经元的持久影响尚不清楚。评估瞬时超声刺激后数小时内神经元的兴奋性特征。用40 s、200 kHz脉冲超声刺激或假刺激刺激原代大鼠皮层神经元。本研究采用全细胞膜片钳技术，通过诱发动作电位对体细胞电流注入的反应来研究神经元的内在放电特性。在超声刺激后的设定时间点进行记录：0-2 h、6-8 h、12-14 h和24-26 h。透射电子显微镜用于评估突触超微结构在相同的时间点。在0-2小时的窗口中，用超声刺激的神经元显示诱发动作电位的平均频率比对照细胞水平增加32%（p = 0.023）。在4-6小时后，测量该增加为44%（p = 0.0043）。到12-14小时，这种效应被消除，并且在刺激后24-26小时仍然不存在。动作电位放电的这些变化与对照组和超声刺激神经元之间在动作电位半宽、去极化率和复极化率方面的统计学显著差异一起发生，这些差异在刺激后24小时同样被消除。这些影响发生在没有改变内在膜特性或突触超微结构。我们报告说，刺激神经元与40秒的超声波增强其兴奋性长达8小时，结合动作电位动力学的修改。这发生在没有主要的超微结构变化或内在膜性质的修改。这些结果可以为经颅超声在实验和治疗环境中的应用提供信息。40 s的超声刺激增强了培养的大鼠神经元的内在兴奋性。增强的兴奋性持续长达8小时。超声对神经元超微结构无影响。
Transcranial ultrasound stimulation can acutely modulate brain activity, but the lasting effects on neurons are unknown. To assess the excitability profile of neurons in the hours following transient ultrasound stimulation. Primary rat cortical neurons were stimulated with a 40 s, 200 kHz pulsed ultrasound stimulation or sham-stimulation. Intrinsic firing properties were investigated through whole-cell patch-clamp recording by evoking action potentials in response to somatic current injection. Recordings were taken at set timepoints following ultrasound stimulation: 0–2 h, 6–8 h, 12–14 h and 24–26 h. Transmission electron microscopy was used to assess synaptic ultrastructure at the same timepoints. In the 0–2 h window, neurons stimulated with ultrasound displayed an increase in the mean frequency of evoked action potentials of 32% above control cell levels (p = 0.023). After 4–6 h this increase was measured as 44% (p = 0.0043). By 12–14 h this effect was eliminated and remained absent 24–26 h post-stimulation. These changes to action potential firing occurred in conjunction with statistically significant differences between control and ultrasound-stimulated neurons in action potential half-width, depolarisation rate, and repolarisation rate, that were similarly eliminated by 24 h following stimulation. These effects occurred in the absence of alterations to intrinsic membrane properties or synaptic ultrastructure. We report that stimulating neurons with 40 s of ultrasound enhances their excitability for up to 8 h in conjunction with modifications to action potential kinetics. This occurs in the absence of major ultrastructural change or modification of intrinsic membrane properties. These results can inform the application of transcranial ultrasound in experimental and therapeutic settings. 40 s of ultrasound stimulation enhances intrinsic excitability of cultured rat neurons. Enhanced excitability lasts for up to 8 h. Ultrasound has no effects on neuronal ultrastructure.
登录
查看更多内容
影响因子：
14.8
作者：
Brewer, Gregory J.;Torricelli, John R.
通讯作者：
Torricelli, John R.
影响因子：
3
作者：
Anderson, William W.;Collingridge, Graham L.
通讯作者：
Collingridge, Graham L.
DOI：
10.1093/brain/awy051
发表时间：
2018-04-01
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Fruscione F;Valente P;Sterlini B;Romei A;Baldassari S;Fadda M;Prestigio C;Giansante G;Sartorelli J;Rossi P;Rubio A;Gambardella A;Nieus T;Broccoli V;Fassio A;Baldelli P;Corradi A;Zara F;Benfenati F
通讯作者：
Benfenati F
DOI：
10.1073/pnas.1607541114
发表时间：
2017-02-07
影响因子：
11.1
作者：
Chereau, Ronan;Saraceno, G. Ezequiel;Nagerl, U. Valentin
通讯作者：
Nagerl, U. Valentin
影响因子：
16.2
作者：
Folloni, Davide;Verhagen, Lennart;Sallet, Jerome
通讯作者：
Sallet, Jerome