Repurposing a SARS-CoV-2 surveillance program for infectious respiratory diseases in a university setting.
Repurposing a SARS-CoV-2 surveillance program for infectious respiratory diseases in a university setting.
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DOI：
10.3389/fpubh.2023.1168551
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发表时间：
2023
影响因子：
5.2
通讯作者：
Dean, Delphine
中科院分区：
文献类型：
--
作者：
King, Kylie L.;Ham, Rachel;Smothers, Austin;Lee, Isaac;Bowie, Tyler;Teetsel, Erika;Peng, Congyue;Dean, Delphine
标准的多重RT-qPCR诊断测试使用鼻咽拭子同时检测各种感染，但市售试剂盒可能价格昂贵且通量有限。此前，我们在临床上验证了SARS-CoV-2的唾液RT-qPCR诊断测试，以在大学校园中提供低成本、高通量和低周转时间的测试。在这里，我们开发了一个呼吸道诊断面板检测SARS-CoV-2，流感A和B在一个单一的唾液样本。与临床结果相比，我们的检测方法对甲型流感样本的准确度为93.5%（43/46的一致结果），对SARS-CoV-2准确度或检测限没有影响。此外，我们的检测方法可以检测合成RNA对照产生的不同病毒浓度的模拟合并感染。我们还证实了甲型流感病毒在室温下唾液中长达5天的稳定性。由于唾液采集不需要专门的拭子或经过培训的临床人员，因此该检测方法的成本低于标准鼻咽拭子呼吸系统面板检测。通过重新利用为COVID-19大流行而开发的实验室基础设施，我们的多重检测可用于扩大呼吸道疾病诊断的可及性，特别是对于在COVID-19大流行期间有效利用唾液检测的社区、学校或大学检测应用。
Standard multiplex RT-qPCR diagnostic tests use nasopharyngeal swabs to simultaneously detect a variety of infections, but commercially available kits can be expensive and have limited throughput. Previously, we clinically validated a saliva-based RT-qPCR diagnostic test for SARS-CoV-2 to provide low-cost testing with high throughput and low turnaround time on a university campus. Here, we developed a respiratory diagnostic panel to detect SARS-CoV-2, influenza A and B within a single saliva sample. When compared to clinical results, our assay demonstrated 93.5% accuracy for influenza A samples (43/46 concordant results) with no effect on SARS-CoV-2 accuracy or limit of detection. In addition, our assay can detect simulated coinfections at varying virus concentrations generated from synthetic RNA controls. We also confirmed the stability of influenza A in saliva at room temperature for up to 5 days. The cost of the assay is lower than standard nasopharyngeal swab respiratory panel tests as saliva collection does not require specialized swabs or trained clinical personnel. By repurposing the lab infrastructure developed for the COVID-19 pandemic, our multiplex assay can be used to provide expanded access to respiratory disease diagnostics, especially for community, school, or university testing applications where saliva testing was effectively utilized during the COVID-19 pandemic.
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影响因子：
3.7
作者：
Ham RE;Smothers AR;Che R;Sell KJ;Peng CA;Dean D
通讯作者：
Dean D
影响因子：
9.4
作者：
Mostafa HH;Carroll KC;Hicken R;Berry GJ;Manji R;Smith E;Rakeman JL;Fowler RC;Leelawong M;Butler-Wu SM;Quintero D;Umali-Wilcox M;Kwiatkowski RW;Persing DH;Weir F;Loeffelholz MJ
通讯作者：
Loeffelholz MJ
影响因子：
4.4
作者：
Davis BM;Foxman B;Monto AS;Baric RS;Martin ET;Uzicanin A;Rainey JJ;Aiello AE
通讯作者：
Aiello AE
影响因子：
--
作者：
Malekifar P;Pakzad R;Shahbahrami R;Zandi M;Jafarpour A;Rezayat SA;Akbarpour S;Shabestari AN;Pakzad I;Hesari E;Farahani A;Soltani S
通讯作者：
Soltani S
DOI：
10.15585/mmwr.mm7049e1
发表时间：
2021-12-10
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Delahoy MJ;Mortenson L;Bauman L;Marquez J;Bagdasarian N;Coyle J;Sumner K;Lewis NM;Lauring AS;Flannery B;Patel MM;Martin ET
通讯作者：
Martin ET