APC/C(FZR-1) Controls SAS-5 Levels To Regulate Centrosome Duplication in Caenorhabditis elegans.
APC/C(FZR-1) Controls SAS-5 Levels To Regulate Centrosome Duplication in Caenorhabditis elegans.
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DOI：
10.1534/g3.117.300260
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发表时间：
2017-12-04
期刊：
影响因子：
--
通讯作者：
Song MH
中科院分区：
文献类型：
--
作者：
Medley JC;DeMeyer LE;Kabara MM;Song MH
As the primary microtubule-organizing center, centrosomes play a key role in establishing mitotic bipolar spindles that secure correct transmission of genomic content. For the fidelity of cell division, centrosome number must be strictly controlled by duplicating only once per cell cycle. Proper levels of centrosome proteins are shown to be critical for normal centrosome number and function. Overexpressing core centrosome factors leads to extra centrosomes, while depleting these factors results in centrosome duplication failure. In this regard, protein turnover by the ubiquitin-proteasome system provides a vital mechanism for the regulation of centrosome protein levels. Here, we report that FZR-1, the Caenorhabditis elegans homolog of Cdh1/Hct1/Fzr, a coactivator of the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, functions as a negative regulator of centrosome duplication in the C. elegans embryo. During mitotic cell division in the early embryo, FZR-1 is associated with centrosomes and enriched at nuclei. Loss of fzr-1 function restores centrosome duplication and embryonic viability to the hypomorphic zyg-1(it25) mutant, in part, through elevated levels of SAS-5 at centrosomes. Our data suggest that the APC/CFZR-1 regulates SAS-5 levels by directly recognizing the conserved KEN-box motif, contributing to proper centrosome duplication. Together, our work shows that FZR-1 plays a conserved role in regulating centrosome duplication in C. elegans.
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影响因子：
21.3
作者：
Habedanck, R;Stierhof, YD;Nigg, EA
通讯作者：
Nigg, EA
APC/C共激活因子从ACM1-CDH1复合物的结构中识别DEGRON识别。
影响因子：
16
作者：
He, Jun;Chao, William C. H.;Zhang, Ziguo;Yang, Jing;Cronin, Nora;Barford, David
通讯作者：
Barford, David
影响因子：
64.8
作者：
Chang L;Zhang Z;Yang J;McLaughlin SH;Barford D
通讯作者：
Barford D
DOI：
10.1083/jcb.201107086
发表时间：
2011-10-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brownlee CW;Klebba JE;Buster DW;Rogers GC
通讯作者：
Rogers GC
影响因子：
9.2
作者：
Delattre, Marie;Canard, Coralie;Gonczy, Pierre
通讯作者：
Gonczy, Pierre