Quantitative Estimation of RNA Transcripts Suggests Persistence of Pax-6 Expression in the Postembryonic Chick Retina
Quantitative Estimation of RNA Transcripts Suggests Persistence of Pax-6 Expression in the Postembryonic Chick Retina
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RNA 转录本的定量估计表明 Pax-6 在胚胎后鸡视网膜中持续表达
DOI：
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发表时间：
1999
影响因子：
2.9
通讯作者：
R. Ariyasu
中科院分区：
文献类型：
--
作者：
S. Bhat;S. Rayner;Cindy M. Huang;R. Ariyasu
Pax-6在眼睛的早期胚胎发育期间表达。关于其在功能成熟视网膜中的表达知之甚少。我们已经检测到Pax-6的成绩单在神经节细胞和无长突细胞层在第3，10，17和31天posthatching和2至3个月大的鸡视网膜原位杂交。定量分析证实了这些观察结果。竞争性RT-PCR结果显示，与3 d视网膜[（1.65 ± 0.48）× 1010/mg组织]相比，17 d视网膜中Pax-6转录本的数量[（0.39 ± 0.13）× 1010/mg组织]显著减少（p < 0.001）。虽然显著低于第3天，但与第17天相比，第31天的视网膜[（0.7 ± 0.16）× 1010/mg组织]和2- 3月龄鸡的视网膜[（0.9 ± 0.28）× 1010/mg组织]含有增加的Pax-6转录本。根据RNA的量，第3天视网膜中Pax-6转录物的数量[（0.45 ± 0.14）× 1010/μg RNA]相对于第17天视网膜[（0.4 ± 0.08）× 1010/μ g RNA]没有显著变化（p = 0.29）。然而，在第31天和2-3个月大时，Pax-6转录本的数量增加[（0.65 ± 0.14）× 1010/μg RNA，（0.65 ± 0.2）× 1010/μg RNA）]。鉴于已知Pax-6表达与不同细胞类型的增殖和出现相关，这些数据表明，神经节和内核细胞层中的细胞类型可以在年轻成人视网膜中长时间保持增殖潜力。
Pax-6 is expressed during early embryonic development of the eye. Very little is known about its expression in the functionally mature retina. We have detected Pax-6 transcripts in the ganglion cell- and amacrine cell layers at days 3, 10, 17 and 31 posthatching and in 2- to 3-month-old chick retina by in situ hybridization. These observations were confirmed by a quantitative analysis. Competitive RT-PCR with a homologous internal control revealed a significant reduction (p < 0.001) in the number of Pax-6 transcripts in day 17 retina [(0.39 ± 0.13) × 1010/mg tissue] compared to day 3 retina [(1.65 ± 0.48) × 1010/mg tissue]. Although significantly lower than at day 3, the day 31 retina [(0.7 ± 0.16) × 1010/mg tissue] and retina from 2- to 3-month-old chicks [(0.9 ± 0.28) × 1010/mg tissue] contained an increased number of Pax-6 transcripts in comparison to day 17. On the basis of the amount of RNA, the number of Pax-6 transcripts in the day 3 retina [(0.45 ± 0.14) × 1010/μg RNA] relative to day 17 retina [(0.4 ± 0.08) × 1010/μg RNA] did not change significantly (p = 0.29). However, at day 31 and at 2–3 months of age an increased number of Pax-6 transcripts [(0.65 ± 0.14) × 1010/μg RNA and (0.65 ± 0.2) × 1010/μg RNA, respectively)] were found. In view of the known association of Pax-6 expression with proliferation and emergence of different cell types, these data suggest that cell types in ganglion and inner nuclear cell layers may retain proliferative potential for an extended period in the young adult retina.
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影响因子：
4.6
作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
通讯作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
影响因子：
30.8
作者：
T. Glaser;L. Jepeal;J. Edwards;S. Young;J. Favor;R. Maas
通讯作者：
T. Glaser;L. Jepeal;J. Edwards;S. Young;J. Favor;R. Maas
影响因子：
10.5
作者：
EPSTEIN, JA;GLASER, T;MAAS, RL
通讯作者：
MAAS, RL
DOI：
10.1073/pnas.87.7.2725
发表时间：
1990-04-01
影响因子：
11.1
作者：
GILLILAND, G;PERRIN, S;BUNN, HF
通讯作者：
BUNN, HF
影响因子：
4.4
作者：
Ten Belecky-Adams;Stanislav I. Tomarev;Hua-Shun;Li;Lynda Ploder;Roderick R. Mdnnes;OlofSundin;Ruben Adler
通讯作者：
Ten Belecky-Adams;Stanislav I. Tomarev;Hua-Shun;Li;Lynda Ploder;Roderick R. Mdnnes;OlofSundin;Ruben Adler