Evaluation of multivalent, functional polymeric nanoparticles for imaging applications.
Evaluation of multivalent, functional polymeric nanoparticles for imaging applications.
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DOI：
10.1021/nn102278w
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发表时间：
2011-02-22
期刊：
影响因子：
17.1
通讯作者：
Anderson CJ
中科院分区：
文献类型：
--
作者：
Shokeen M;Pressly ED;Hagooly A;Zheleznyak A;Ramos N;Fiamengo AL;Welch MJ;Hawker CJ;Anderson CJ
以链端功能化的GRGDS肽靶向序列（基于整合素αvβ3的模型体系）为负载，在0 ~ 50%范围内，合成了一系列具有诊断/成像单元和靶向配体的多价功能性聚合物纳米颗粒。在这些系统中，精确的结构和官能团控制是通过模块化的方法实现的，包括使用多个功能化的大单体/单体单元结合活性自由基聚合。细胞实验结果显示，αvβ3整合素阳性的U87MG胶质母细胞瘤细胞对αvβ3整合素的摄取随着RGD负荷的增加而增加，这可能是RGD肽靶向αvβ3整合素的有效性的上限。值得注意的是，这种靶向效率的提高与体内生物分布结果相结合，体内生物分布结果显示，随着RGD负荷的增加，血液循环减少，肝脏摄取增加。结果证明了控制配体负载的重要性，以便在多价纳米颗粒基系统的治疗和成像应用中实现最佳性能。
A series of multivalent, functional polymer nanoparticles with diagnostic/imaging units and targeting ligands for molecular targeting were synthesized with the loading of the chain end functionalized, GRGDS peptide targeting sequence (model system based on integrin αvβ3) ranging from 0 to 50%. Accurate structural and functional group control in these systems was achieved through a modular approach involving the use of multiple functionalized macromonomer/monomer units combined with living free radical polymerization. In cellulo results show an increase in uptake in αvβ3 integrin-positive U87MG glioblastoma cells with increasing RGD loading and a possible upper limit on the effectiveness of the number of RGD peptides for targeting αvβ3 integrin. Significantly, this increased targeting efficiency is coupled with in vivo biodistribution results which show decreased blood circulation and increased liver uptake with increasing RGD loading. The results demonstrate the importance of controlling ligand loading in order to achieve optimal performance for therapeutic and imaging applications for multivalent nanoparticle based systems.
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使用简单的比色和高灵敏度双光子散射测定法，基于多功能椭圆形金纳米粒子选择性检测乳腺癌细胞。
影响因子：
17.1
作者：
Lu, Wentong;Arumugam, Ranjini;Senapati, Dulal;Singh, Anant K.;Arbneshi, Tahir;Khan, Sadia Afrin;Yu, Hongtao;Ray, Paresh Chandra
通讯作者：
Ray, Paresh Chandra
DOI：
10.1002/1099-0518(200012)38:1
发表时间：
2000-01-01
影响因子：
--
作者：
Fraser, CL;Smith, AP
通讯作者：
Smith, AP
影响因子：
5.5
作者：
Alb, Alina M.;Enohnyaket, Pascal;Reed, Wayne F.
通讯作者：
Reed, Wayne F.
影响因子：
5.5
作者：
Kilcher, Giona;Wang, Lei;Tirelli, Nicola
通讯作者：
Tirelli, Nicola
影响因子：
15
作者：
Parrish, B;Breitenkamp, RB;Emrick, T
通讯作者：
Emrick, T