Single-cell spatial immune landscapes of primary and metastatic brain tumours.
Single-cell spatial immune landscapes of primary and metastatic brain tumours.
复制标题
DOI：
10.1038/s41586-022-05680-3
复制
发表时间：
2023-02
期刊：
影响因子：
64.8
通讯作者：
Walsh, Logan A.
中科院分区：
文献类型：
--
作者：
Karimi, Elham;Yu, Miranda W. W.;Maritan, Sarah M.;Perus, Lucas J. M.;Rezanejad, Morteza;Sorin, Mark;Dankner, Matthew;Fallah, Parvaneh;Dore, Samuel;Zuo, Dongmei;Fiset, Benoit;Kloosterman, Daan J.;Ramsay, LeeAnn;Wei, Yuhong;Lam, Stephanie;Alsajjan, Roa;Watson, Ian R.;Roldan Urgoiti, Gloria;Park, Morag;Brandsma, Dieta;Senger, Donna L.;Chan, Jennifer A. A.;Akkari, Leila;Petrecca, Kevin;Guiot, Marie-Christine;Siegel, Peter M.;Quail, Daniela F.;Walsh, Logan A.
单细胞技术使肿瘤微环境的表征达到了前所未有的深度，并揭示了肿瘤细胞及其生态位之间巨大的细胞多样性。抗肿瘤免疫依赖于肿瘤微环境中的细胞-细胞关系，但许多单细胞研究缺乏空间背景，依赖于解离的组织。在这里，我们应用成像质谱细胞术来表征139例高级别胶质瘤和46例脑转移瘤患者的免疫学特征。对389个高维组织病理学图像中超过110万个细胞的单细胞分析实现了免疫谱系和激活状态的空间分辨率，揭示了原发性肿瘤和不同实体癌脑转移瘤之间的免疫景观差异。这些分析揭示了与胶质母细胞瘤患者生存相关的细胞邻域，我们利用这些细胞邻域来鉴定与长期生存相关的髓过氧化物酶（MPO）阳性巨噬细胞的独特群体。我们的研究结果提供了对原发性和转移性脑肿瘤生物学的深入了解，加强了将空间分辨率整合到单细胞数据集以解剖癌症微环境结构的价值。人脑肿瘤的成像质量细胞计数提供了空间信息，结合现有的转录组学数据，揭示了存在一个包含与延长生存期相关的罕见巨噬细胞群体的细胞邻域。
Single-cell technologies have enabled the characterization of the tumour microenvironment at unprecedented depth and have revealed vast cellular diversity among tumour cells and their niche. Anti-tumour immunity relies on cell–cell relationships within the tumour microenvironment, yet many single-cell studies lack spatial context and rely on dissociated tissues. Here we applied imaging mass cytometry to characterize the immunological landscape of 139 high-grade glioma and 46 brain metastasis tumours from patients. Single-cell analysis of more than 1.1 million cells across 389 high-dimensional histopathology images enabled the spatial resolution of immune lineages and activation states, revealing differences in immune landscapes between primary tumours and brain metastases from diverse solid cancers. These analyses revealed cellular neighbourhoods associated with survival in patients with glioblastoma, which we leveraged to identify a unique population of myeloperoxidase (MPO)-positive macrophages associated with long-term survival. Our findings provide insight into the biology of primary and metastatic brain tumours, reinforcing the value of integrating spatial resolution to single-cell datasets to dissect the microenvironmental contexture of cancer. Imaging mass cytometry of human brain tumours provides spatial information that, combined with existing transcriptomic data, reveals the existence of a cellular neighbourhood containing a rare macrophage population associated with prolonged survival.
登录
查看更多内容
影响因子：
22.7
作者：
Ali, H. Raza;Jackson, Hartland W.;Bodenmiller, Bernd
通讯作者：
Bodenmiller, Bernd
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
82.9
作者：
Binnewies M;Roberts EW;Kersten K;Chan V;Fearon DF;Merad M;Coussens LM;Gabrilovich DI;Ostrand-Rosenberg S;Hedrick CC;Vonderheide RH;Pittet MJ;Jain RK;Zou W;Howcroft TK;Woodhouse EC;Weinberg RA;Krummel MF
通讯作者：
Krummel MF
影响因子：
5.2
作者：
Dankner M;Lam S;Degenhard T;Garzia L;Guiot MC;Petrecca K;Siegel PM
通讯作者：
Siegel PM
影响因子：
64.5
作者：
Klemm F;Maas RR;Bowman RL;Kornete M;Soukup K;Nassiri S;Brouland JP;Iacobuzio-Donahue CA;Brennan C;Tabar V;Gutin PH;Daniel RT;Hegi ME;Joyce JA
通讯作者：
Joyce JA