Mutagenesis of the Herpesvirus Saimiri Terminal Repeat Region Reveals Important Elements for Virus Production
Mutagenesis of the Herpesvirus Saimiri Terminal Repeat Region Reveals Important Elements for Virus Production
复制标题
疱疹病毒 Saimiri 末端重复区的诱变揭示了病毒产生的重要元素
DOI：
10.1128/jvi.02579-06
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发表时间：
2007
影响因子：
5.4
通讯作者：
M. Allday
中科院分区：
文献类型：
--
作者：
Robert E. White;Lindsay Carline;M. Allday
猴疱疹病毒（HVS）末端重复序列（TR）的缺失使其无法产生感染性病毒或产生斑块。然而，tr缺失的HVS细菌人工染色体可以形成复制区室。该突变体的互补表明，一个TR拷贝加上基因组与TR的正确连接，足以有效地形成空斑和产生传染性病毒。在TR单元内，基因组切割位点周围的区域似乎是病毒产生的必要和充分条件。对产生细胞的片段分析表明，在裂解周期中有扩增TR数的倾向。
ABSTRACT Deletion of the terminal repeats (TR) from herpesvirus saimiri (HVS) renders it unable to produce infectious virus or generate plaques. However, a TR-deleted HVS bacterial artificial chromosome can form replication compartments. Complementation of this mutant shows that one copy of the TR, plus the right junction of the genome with the TR, is sufficient for efficient plaque formation and generation of infectious virus. Within the TR unit, the region around the cleavage site of the genome appears both necessary and sufficient for virus production. Analysis of episomes from productive cells indicates a propensity to amplify TR numbers during the lytic cycle.
影响因子：
56.9
作者：
Ballestas, ME;Chatis, PA;Kaye, KM
通讯作者：
Kaye, KM
影响因子：
3.8
作者：
Collins, CM;Medveczky, MM;Medveczky, PG
通讯作者：
Medveczky, PG