Recently Integrated Alu Elements in Capuchin Monkeys: A Resource for Cebus/Sapajus Genomics.
Recently Integrated Alu Elements in Capuchin Monkeys: A Resource for Cebus/Sapajus Genomics.
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DOI：
10.3390/genes13040572
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发表时间：
2022-03-24
期刊：
影响因子：
3.5
通讯作者：
Batzer, Mark A.
中科院分区：
文献类型：
--
作者：
Storer, Jessica M.;Walker, Jerilyn A.;Rockwell, Catherine E.;Mores, Grayce;Beckstrom, Thomas O.;Orkin, Joseph D.;Melin, Amanda D.;Phillips, Kimberley A.;Roos, Christian;Batzer, Mark A.
卷尾猴是鸭嘴猴科（美洲发现的猴子）。在大部分分类学历史中，卷尾猴的两种主要形态类型，纤细型（未簇绒的）和粗壮型（簇绒的），被归为一个属，Cebus。此外，所有簇绒卷尾猴都被归入同一个物种，即 Cebus apella，尽管其地理范围横跨南美洲中部和北部。 2012 年，簇绒卷尾猴被归入 Sapajus 属，目前已确认有 8 个物种和 5 个 Cebus 物种，尽管这些数字仍存在争议。 Alu 逆转录转座子是一类广泛用于研究灵长类系统发育的移动元件插入 (MEI)。然而，铝元素很少被用来研究卷尾猴。最近的卷尾猴基因组水平组装（Cebus imitator; [Cebus_imitator_1.0] 和 Sapajus apella [GSC_monkey_1.0]）促进了大规模确定年轻谱系特异性 Alu 插入。这里报告了 1607 个卷尾猴特异性和 678 个 Sapajus 特异性 Alu 插入以及用于许多元素的位点特异性 PCR 测定的候选寡核苷酸。 PCR 分析鉴定出 104 个属水平和 51 个种水平 Alu 插入多态性。本研究报告的 Alu 数据集提供了宝贵的资源，将有助于对缺乏表型数据的档案样本进行分类以及卷尾猴系统发育关系的研究。
Capuchins are platyrrhines (monkeys found in the Americas) within the Cebidae family. For most of their taxonomic history, the two main morphological types of capuchins, gracile (untufted) and robust (tufted), were assigned to a single genus, Cebus. Further, all tufted capuchins were assigned to a single species, Cebus apella, despite broad geographic ranges spanning Central and northern South America. In 2012, tufted capuchins were assigned to their genus, Sapajus, with eight currently recognized species and five Cebus species, although these numbers are still under debate. Alu retrotransposons are a class of mobile element insertion (MEI) widely used to study primate phylogenetics. However, Alu elements have rarely been used to study capuchins. Recent genome-level assemblies for capuchins (Cebus imitator; [Cebus_imitator_1.0] and Sapajus apella [GSC_monkey_1.0]) facilitated large scale ascertainment of young lineage-specific Alu insertions. Reported here are 1607 capuchin specific and 678 Sapajus specific Alu insertions along with candidate oligonucleotides for locus-specific PCR assays for many elements. PCR analyses identified 104 genus level and 51 species level Alu insertion polymorphisms. The Alu datasets reported in this study provide a valuable resource that will assist in the classification of archival samples lacking phenotypic data and for the study of capuchin phylogenetic relationships.
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影响因子：
4.4
作者：
BATZER, MA;DEININGER, PL
通讯作者：
DEININGER, PL
影响因子：
4.1
作者：
Lima, Marcela G. M.;de Sousa e Silva-Junior, Jose;Alfaro, Jessica W. Lynch
通讯作者：
Alfaro, Jessica W. Lynch
影响因子：
3.3
作者：
Konkel MK;Walker JA;Hotard AB;Ranck MC;Fontenot CC;Storer J;Stewart C;Marth GT;1000 Genomes Consortium;Batzer MA
通讯作者：
Batzer MA
DOI：
10.1590/0001-3765202120190802
发表时间：
2021-01-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
MORAES, BÁRBARA L.C. DE;BORGES, DANIELA B.;BEZERRA, BRUNA
通讯作者：
BEZERRA, BRUNA
影响因子：
2.4
作者：
Martins-, Antonio M. G., Jr.;Amorim, Nadia;Schneider, Horacio
通讯作者：
Schneider, Horacio