Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals.
Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals.
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DOI：
10.7150/thno.3459
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发表时间：
2012
期刊：
影响因子：
12.4
通讯作者：
Prasad PN
中科院分区：
文献类型：
--
作者：
Law WC;Mahajan SD;Kopwitthaya A;Reynolds JL;Liu M;Liu X;Chen G;Erogbogbo F;Vathy L;Aalinkeel R;Schwartz SA;Yong KT;Prasad PN
将诊断成像和治疗功能集成到单一系统中的治疗诊断平台已成为纳米颗粒研究的新方向。在治疗过程中，监测疗效。治疗诊断纳米颗粒的使用可以添加额外的“层”来跟踪治疗剂，例如药代动力学和生物分布。在本报告中，我们开发了基于量子棒 (QR) 的制剂，用于将小干扰 RNA (siRNA) 递送至人类神经元细胞。具有不同表面官能团（胺和马来酰亚胺）的聚乙二醇化QR被设计用于选择性下调与药物滥用行为相关的多巴胺能信号通路。我们已经证明，DARPP-32 siRNA 已成功递送至多巴胺能神经元 (DAN) 细胞，从而通过静电和共价键缀合方案大幅降低特定基因表达。聚乙二醇化表面为 QR 制剂提供了高生物相容性和可忽略不计的细胞毒性，这可能有助于这些纳米颗粒的体内应用。
Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep track on the therapeutic agent such as the pharmacokinetics and biodistribution. In this report, we have developed quantum rod (QR) based formulations for the delivery of small interfering RNAs (siRNAs) to human neuronal cells. PEGlyated QRs with different surface functional groups (amine and maleimide) were designed for selectively down-regulating the dopaminergic signaling pathway which is associated with the drug abuse behavior. We have demonstrated that the DARPP-32 siRNAs were successfully delivered to dopaminergic neuronal (DAN) cells which led to drastic knockdown of specific gene expression by both the electrostatic and covalent bond conjugation regimes. The PEGlyated surface offered high biocompatibilities and negligible cytotoxicities to the QR formulations that may facilitate the in vivo applications of these nanoparticles.
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